Abstract：Objective To investigate the effect and mechanism of targeting protein for Xklp2 gene on suppressing the proliferation and apoptosis of breast cancer cells by regulating p38MAPK signaling pathway. Methods The expression of TPX2 gene in breast cancer tissue and paracancerous tissue was detected by Western blot. siRNA-NC, TPX2-siRNA1 and TPX2-siRNA2 were transfected into human breast cancer MCF-7 cells, and the cells without any siRNA transfection served as the control group. The expression of TPX2 protein in the transfected cells in each siRNA transfection group was detected by Western blot. 48-hour after cell transinfection, the proliferation of cells in siRNA-NC-transfected group and TPX2-siRNA-transfected group was detected by CCK8 assay. The cell apoptosis was detected by flow cytometry. The expression of Cleaved caspase3, p38 and p-p38 proteins was detected by Western blot. Results The expression of TPX2 in breast cancer tissues was significantly higher than that in adjacent tissues (P<0.01). The expression of TPX2 protein in the cells of TPX2-siRNA1-transfected group and TPX2-siRNA2-transfected group was significantly lower than that of the control group (P<0.01). The inhibitory effect of TPX2-siRNA2-transfected group was more obvious, which was selected in the following study. The cell survival rate, the apoptosis rate and the expression of Cleaved caspase3 and p-p38 proteins showed no statistically significant differences between siRNA-NC-transfected group and the control group (all P>0.05). The cell survival rate and the expression of p-p38 protein in TPX2-siRNA-transfected group were significantly lower than those of the control group, while the apoptosis rate and the expression of Cleaved caspase3 protein were significantly higher (P<0.01). The expression of p38 protein showed no statistically significant difference among the three groups (P>0.05). Conclusions TPX2 gene is highly expressed in breast cancer tissues. The inhibition of TPX2 expression can reduce cell proliferation and promote cell apoptosis, and its mechanism is related to the activation of p38MAPK signaling pathway.
吴池华, 李一, 杨麟翰, 黄婷, 喻渊. TPX2基因调控p38MAPK信号通路抑制乳腺癌细胞增殖及促进凋亡的机制研究[J]. 实用预防医学, 2017, 24(8): 941-945.
WU Chi-hua, LI Yi, YANG Lin-han, HUANG Ting, YU Yuan. Mechanism of TPX2 gene on restraining the proliferation and promoting apoptosis of breast cancer cells by regulating p38MAPK signaling pathway. , 2017, 24(8): 941-945.
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2017, Vol. 24 
