青海地区藏族人群IFN-γ、TNF-α及IL-10基因多态性与乙型肝炎病毒感染易感性的关系

doi:10.3969/j.issn.1006-3110.2017.07.014

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摘要目的探讨乙型病毒性肝炎易感性与IFN-γ、TNF-α及IL-10基因单核苷酸多态性的相关性,旨在基因层面确立青海地区藏族人群HBV易感性的影响因素。方法收集来青海大学附属医院和青海省第四人民医院就诊的藏族人群HBV感染患者血样258例作为病例组,从体检中心收集的健康人群血样126例作为对照组。DNA提取试剂盒提取外周血基因组DNA,用飞行质谱技术检测IFN-γ(+874A/T、+2109C/T)、TNF-α(-308)、IL-10(-592,-1082)多态性位点的碱基及基因型,并分析与乙型肝炎病毒性易感性的相关性。结果 TNF-α(-308)和IL-10(-592,-1082)多态性位点的碱基及基因型频率分布在病例组(92.4%、7.6%;24%、52%、24%, 1.2%、23.2%、75.6%)与对照组(92.5%、7.5%;23.9%、46.3%、29.8%, 4.5%、19.4%、76.1%)分布差异无统计学意义(P>0.05)。尽管IFN-γ多态性位点+2109C/T的基因型和碱基及IFN-γ+874A/T位点的基因型在对照组(14.3%、43.7%、42%;2.4%、19.8%、77.8%)和病例组(10.5%、47.3%、42.2%;3.1%、30.6%、66.3%)的频率分布差异无统计学意义(P>0.05),但IFN-γ+874A/T位点的A碱基在病例组的频率(18.4%)分布显著高于对照组(12.3%)(P<0.05),T碱基在对照组的频率(87.7%)分布显著高于病例组(81.6%),差异有统计学意义(P<0.05)。结论在青海藏族人群,IFN-γ+874A/T位点的等位基因T可能是HBV感染的保护性因素,而等位基因A是HBV感染的易感因素。

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	任利
	路越晴
	程冠
	于文昊
	王芝
	王聪
	鄂伟健
	王海久
	周瀛
	阳丹才让
	侯立朝
	樊海宁

关键词 ：干扰素-γ, 肿瘤坏死因子-α, 白介素-10, 乙肝, 基因多态性

Abstract：Objective To explore the genetic correlations between the susceptibility to hepatitis B virus (HBV) and single nucleotide polymorphisms of IFN-γ, TNF-α and IL-10 so as to identify the influencing factors of HBV susceptibility at the genetic level in Tibetan population in Qinghai area. Methods We collected 258 blood samples from Tibetan patients infected with HBV in the Affiliated Hospital of Qinghai University and the Fourth People’s Hospital of Qinghai Province to serve as the case group. And 126 blood samples from healthy individuals in the Physical Examination Center were collected as the control group. Genomic DNA was extracted from the peripheral blood by DNA extraction kit. The bases and genotypes of gene polymorphism loci of IFN-γ (+874A/T, +2 109C/T), TNF-α (-308) and IL-10 (-592, -1,082) were detected by flight mass spectrometry, and their correlations with the susceptibility to hepatitis B virus were analyzed. Results No statistically significant differences were found in the frequency distribution of bases and genotypes of gene polymorphism loci of TNF-α (-308) and IL-10 (-592, -1 082) between the case group (92.4%, 7.6%; 24%, 52%, 24%; 1.2%, 23.2%, 75.6%) and the control group (92.5%, 7.5%; 23.9%, 46.3%, 29.8%; 4.5%, 19.4%, 76.1%; all P>0.05). Although the frequency distribution of bases and genotypes of IFN-γ +2109C/T and IFN-γ +874A/T polymorphism loci in the control group (14.3%, 43.7%, 42%; 2.4%, 19.8%, 77.8%) and the case group (10.5%, 47.3%, 42.2%; 3.1%, 30.6%, 66.3%) showed no statistically significant differences (allP>0.05), but the frequency distribution of base A of IFN- γ+874A/T loci was significantly higher in the case group than in the control group (18.4% vs.12.3%) and base T was significantly higher in the control group than in the case group (87.7%vs.81.6%) (both P<0.05).Conclusions For the Tibetan population in Qinghai Province, the allele T of IFN-γ+874A/T may be the protective factor of HBV infection, while the allele A is the predisposing factor of HBV infection.

Key words： IFN-γ TNF-α interleukin-10 HBV infection gene polymorphism

收稿日期: 2017-01-01

R181.2+4

基金资助:国家自然科学基金资助项目(81060133)

通讯作者: 樊海宁,E-mail:fanhaining@medmail.com.cn。

作者简介: 任利(1976-),男,甘肃张掖人,硕士,副主任医师、副教授,主要从事肝胆胰疾病方面研究工作。

引用本文:

任利, 路越晴, 程冠, 于文昊, 王芝, 王聪, 鄂伟健, 王海久, 周瀛, 阳丹才让, 侯立朝, 樊海宁. 青海地区藏族人群IFN-γ、TNF-α及IL-10基因多态性与乙型肝炎病毒感染易感性的关系[J]. 实用预防医学, 2017, 24(7): 814-818. REN Li, LU Yue-qing, CHENG Guan, YU Wen-hao, WANG Zhi, WANG Cong, E Wei-jian,WANG Hai-jiu, ZHOU Ying, YANGDAN Cai-rang, HOU Li-zhao, FAN Hai-ning. Association between the susceptibility of HBV infection and gene polymorphismsof IFN-γ, TNF-α and IL-10 in the Tibetan population in Qinghai area. , 2017, 24(7): 814-818.

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[1] 高金明, 林耀广, 邱长春,等. 肿瘤坏死因子α/淋巴毒素α基因多态性与支气管哮喘的关系[J] . 基础医学与临床, 2003, 23(5):512-516.
[2] 李金强, 蒋道荣, 姚登福,等. 乙型肝炎患者血清IL-18水平的检测及其临床意义[J] . 交通医学, 2006, 20(1):51-53.
[3] 张平安, 吴健民, 李艳,等. 汉族人白细胞介素-18基因启动子多态性与慢性乙型肝炎的相关性研究[J] . 中华医学遗传学杂志, 2005, 22(5):528-532.
[4] 高建梅, 邹云莲, 董虹,等. 云南三民族人群IL-18启动子基因多态性与HBV感染的相关性研究[J] . 中华临床医师杂志:电子版, 2010, 4(9):46-51.
[5] 张健, 林华, 李卫宁. IL-18基因编码区105位点多态性及其血清含量相关性研究[J] . 现代检验医学杂志, 2006, 21(1):40-42.
[6] 孙心岩. HLA基因多态性与乙肝相关肝细胞癌易患性关系的研究[D] . 上海:第二军医大学, 2012.
[7] 王春艳, 蒋利萍. 白细胞介素18基因多态性与疾病的关系研究进展[J] . 检验医学与临床, 2007, 4(2):104-106.
[8] Cheong JY, Cho SW, Hwang IL, et al. Association between chronic hepatitis B virus infection and interleukin-10, tumor necrosis factor- gene promoter polymorphisms[J] . J Gastroenterol Hepatol, 2006, 21(7):1163-1169.
[9] Bellamy R, Ruwende C, Corrah T, et al. Tuberculosis and chronic hepatitis B virus infection in Africans and variation in the vitamin D receptor gene[J] . J Infect Dis, 1999,179(3):721-724.
[10] 孔令杰. 系统性红斑狼疮的基因、环境因素以及基因—环境交互作用研究[D] . 上海:复旦大学, 2008.
[11] 王博蔚.肿瘤坏死因子基因多态性与炎症[J] . 国外医学免疫学分册,2000,23(2):89.
[12] Bozkaya H, Bozdayi MR, Sarioglu M, et al. Circulating IL-2, IL-10 and TNF-alpha in chronic hepatitis B: their relations to HBeAg status and the activity of liver disease[J] . Hepatogastroenterology, 2000, 47(36):1675-1679.
[13] Biermer M, Puro R, Schneider RJ. Tumor necrosis factor alpha inhibition of hepatitis B virus replication involves disruption of capsid integrity through activation of NF-kappa B[J] . J Virol, 2003, 77(7):4033-4042.
[14] Allen RD. Polymorphism of the human TNF-alpha promoter--random variation or functional diversity[J] . Mol Immunol, 1999, 36(15-16):1017-1027.
[15] Miyazoe S, Hamasaki K, Nakata K, et al. Influence of interleukin-10 gene promoter polymorphisms on disease progression in patients chronically infected with hepatitis B virus[J] . Am J Gastroenterol, 2002, 97(8):2086-2092.
[16] Tsuchiya N, Tokushige K, Yamaguchi N, et al. Influence of TNF gene polymorphism in patients with acute and fulminant hepatitis[J] . J Gastroenterol, 2004, 39(9):859-866.
[17] Ben-Ari Z, Mor E, Papo O, et al. Cytokine gene polymorphisms in patients infected with hepatitis B virus[J] . Am J Gastroenterol, 2003, 98(1):144-150.
[18] H hler T, Kruger A, Gerken G, et al. A tumor necrosis factor-alpha(TNF-alpha) promoter polymorphism is associated with chronic hepatitis B infection[J] . Clin Exp Immunol, 1998, 111(3):579-582.
[19] 张平安, 李艳, 向萍霞. 肿瘤坏死因子α基因启动子多态性与乙型肝炎易感性的相关性分析[J] . 检验医学, 2004, 19(6):493-496.