Abstract：Objective To investigate the antibiotic resistance of Acinetobacter baumannii (A. baumannii) and the carriage of resistance genes of carbapenem-resistant Acinetobacter baumannii (CRAB) isolated from two hospitals in Zhongshan city, to analyze their gene homology, and to provide evidence for nosocomial infection control. Methods Thirty-five clinical isolates of A. baumannii were collected from two hospitals in Zhongshan city from March to August 2018. Susceptibilities of the Acinetobacter baumannii isolates to 16 kinds of antibiotics were detected by minimum inhibitory concentration (MIC) method. The resistance genes for the selected CRAB isolates were determined by polymerase chain reaction (PCR) and DNA sequencing. Molecular genotyping was performed for 35 Acinetobacter baumannii isolates by pulsed-field gel electrophoresis (PFGE). Results Thirty-three CRAB isolates were screened from 35 A. baumannii isolates, and the other 2 isolates were sensitive to all the 16 kinds of antibiotics. 33 CRAB isolates were sensitive to tigecycline and polymyxin B, and their sensitivity rates to minocycline and other antibiotics were 45.45% and above 80%, respectively. All the 33 CRAB isolates carried OXA-51 gene, but IMP and VIM genes were not detected. 18 PFGE types and 6 clusters(A-F) were identified in the 35 A. baumannii isolates. Cluster A (9/35, 25.7%) and cluster B (22/35, 62.9%) were the main prevalent clones. Conclusions Antibiotic resistance of the A. baumannii isolates in two hospitals in Zhongshan city is serious, and carrying various drug resistance genes may be the important reason for the resistance of A.baumannii to carbapenems and other antibacterial drugs. There are different types of clone isolates scattered in the two hospitals and the same clone isolate prevailed and spread between the two hospitals.
2020, Vol. 27 
