Antibiotic resistance and PFGE subtyping of foodborne Salmonella C-group in Hunan Province, 2010-2016
CHEN Shuai, JIA Hua-yun, WANG Lan, ZHAN Zhi-fei, ZHANG Hong, CAI Liang
Key Laboratory of Microbial Molecular Biology of Hunan Province, Hunan Provincial Center for Disease Control and Prevention, Changsha, Hunan 410005, China
Abstract：Objective To investigate the drug resistance and molecular typing characteristics of foodborne Salmonella C-group in Hunan Province. Methods A sensitive test of 7 antimicrobial agents was carried out by using micro-broth dilution method in 80 strains of C-group Salmonella isolated from food in Hunan Province during 2010-2016, and pulsed-field gel electrophoresis (PFGE) was used for the molecular typing. Results Among 80 strains of Salmonella C-group, 30.00% (24/80) strains were sensitive to all antimicrobial agents, and the remaining 70.00% (56/80) strains were resistant to at least 1 antimicrobial agent. There were 33.75% (27/80) strains simultaneously multiple resistant to three or more antimicrobial agents. Analysis of PFGE pattern cluster atlas in 80 strains of Salmonella obtained 61 types, and the genetic similarity coefficient was in the range of 55.3%-100%, with 1-7 strains per type. Conclusions The drug resistance of Salmonella C-group in Hunan Province during 2010-2016 is severe. To establish a perfect background database concerning molecular classification of pathogens will improve the ability of early warning of foodborne disease outbreaks.
2017, Vol. 24 
