Study on drug resistance genes of aminoglycosides modifying enzymes among Klebsiella pneumoniae
YE chun-mei1,2,LIU wen-en1
1 Xiangya Hospital of Central South University Changsha 410008; 2 The traditional hospital of Changsha(the eighth hospital of Changsha).Changsha 410001
Abstract：ObjectiveTo investigate the drug resistance and detect aminoglycosides modifying enzymes in Klebsiella pneumonia from Xiangya Hospital of Central South University. Methods96 non-repetitive clinical isolates of Klebsiella pneumoniae strains were collected from Xiangya hospitals of Central South University in January 2009 to July 2009. aminoglycosides modifying enzymes（aac(3)-Ⅰ、aac(6′)-Ⅰb、ant(3″)-Ⅰ、 aph(3′)-Ⅵa）was detected by PCR.. ResultAmong aminoglycosides modifying enzymes isolates of Klebsiella pneumoniae through PCR amplification,aac(3)-Ⅰ、aac(6′)-Ⅰb and ant(3″)-Ⅰ isolates accounted for 18.8%、57.3% and 3.1% respectively.aac(6′)-Ⅰb is the most common genotype.The prevalence of aac(6′)-Ⅰb in ciprofloxacin-resistant and susceptible Isolates were 79.1%（19/24）and 50%（36/72）. Through Chi-Square Tests , the difference was statistically significant（χ2=6.26,P<0.05）. Conclusionaac(3)-Ⅰ、aac(6′)-Ⅰb、ant(3″)-Ⅰ aminoglycosides modifying enzymes had emerged for the first time in Human Province , aac(6′)-Ⅰb is the most common genotype. It may play an important role in Aminoglycosides resistance.
叶春枚,刘文恩. 肺炎克雷伯菌氨基糖苷类修饰酶耐药基因研究[J]. 实用预防医学, 2015, 22(7): 863-865.
YE chun-mei,LIU wen-en. Study on drug resistance genes of aminoglycosides modifying enzymes among Klebsiella pneumoniae. , 2015, 22(7): 863-865.
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2015, Vol. 22 
