Abstract：ObjectiveTo develop a method for determination Cadmium（Ca） and Phosphorus （P）in human Milk, and to investigate the contents of Ca and P in human milk in Shenzhen. Methods221 human milk (1~2 months postpartum) samples were collected and preserved after lyophilization in Shenzhen during the year of 2011 to 2013. After high-pressure digestion by HNO3 in closed-vessel, the elements of Ca and P were simultaneously determined by ICP-OES. ResultsThe detection limits of Ca and P were 0.088mg/kg and 0.024mg/kg respectively. The average correlation coefficients were all ≥0.9999 with good linearity, the relative standard deviation of Ca and P ranged from 1.3% to 2.5％ and from 1.6% to 2.1％ respectively,the results of analyzing standard materials were all in the certificate values. The level of Ca in human milk from Shenzhen ranged from 187.6mg/kg to 449.6mg/kg and the average concentration was 294.5mg/kg, the level of P ranged from 84.9mg/kg to 230.7mg/kg and the average concentration was 157.7mg/kg, the ratio of Ca and P ranged from 1.1 to 3.7 and the average value was 1.9. ConclusionsThe method has high accuracy and good reproducibility, which could be used to determinate the elements of Ca and P in human milk. The average content of Ca in the human milk from Shenzhen was within the reference ranges provided by the World Health Organization (WHO), the average content of P exceeded slightly the reference ranges provided by the World Health Organization (WHO).
姜杰,张慧敏,李胜浓,蒋友胜,周健,林凯,丘红梅,张建清. 电感耦合等离子体发射光谱法测定深圳市母乳中钙和磷[J]. 实用预防医学, 2015, 22(8): 915-917.
JIANG Jie, ZHANG Huimin, LI Shengnong, et al.. The Determination of Cadmium and Phosphorus of Human Milk in Shenzhen by Inductively Coupled Plasma Optical Emission Spectrometer. , 2015, 22(8): 915-917.
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