Abstract：Objective To analyze the laboratory diagnosis of cases of imported Plasmodium malariae infection so as to reduce its misdiagnosis and missed diagnosis. Methods The cases of imported Plasmodium malariae infection were confirmed by morphological test of thick and thin blood smears and nested polymerase chain reaction (PCR) malaria detection assay. Results The results of morphological test and nested PCR malaria detection assay showed that 7 cases of Plasmodium malariae infection which were initially diagnosed with non-Plasmodium infection, Plasmodium infection and Plasmodium falciparum infection were detected, including 1 case of mixed Plasmodium malariae and Plasmodium vivax infections. Conclusions The training about morphological diagnosis of Plasmodium species and the related molecular biology techniques should be continuously strengthened and promoted in malaria eliminated areas so as to meet the correct diagnosis and species identification of imported malaria cases at the present stage, especially in rare and seldom species identification.
[1] 卫生部地方病防治局. 疟疾防治手册[M].第3版. 北京:人民卫生出版社,2007:70-78. [2] 张兵,田斌,廖瑜,等. 巢式PCR在输入性疟疾诊断和分型中的应用价值[J]. 实用预防医学,2013,20(2):229-231. [3] 田斌,段绩辉,徐明忠,等. 三例输入性卵形疟的实验室诊断[J]. 检验医学,2014,29(8):851-855. [4] 魏沨,万正,韩墨,等. 非洲输入性三日疟2例[J]. 中国血吸虫病防治杂志,2014,26(2):232-233. [5] Carcia LS. Diagnostic medical parasitology[M]. 5th ed. Washington,DC:ASM Press,2006. [6] 张进顺,高兴政. 临床寄生虫检验学[M]. 北京:人民卫生出版社,2009:105-108. [7] Baltzell KA, Shakely D, Hsiang M, et al. Prevalence of PCR detectable malaria infection among febrile patients with a negative Plasmodium falciparum specific rapid diagnostic test in Zanzibar[J]. Am J Trop Med Hyg,2013,88(2):289-291. [8] 邓艳,周瑞敏,张红卫,等. 河南省3例输入性三日疟的诊治分析[J]. 中国寄生虫学与寄生虫病杂志,2014,32(1):61-63. [9] Andrade BB, Reis-Filho A, Barros AM, et al. Towards a precise test for malaria diagnosis in the Brazilian Amazon: comparison among field microscopy, a rapid diagnostic test, nested PCR, and a computational expert system based on artificial neural networks[J]. Malar J,2010,9:117. [10] Chong C, Cho PY, Na B, et al. Evaluation of the accuracy of the easy test malaria Pf/Pan Ag, a rapid diagnostic test, in Uganda[J]. Korean JParasitol,2014,52(5):501-505. [11] Baiden F, Owusu-Agyei S, Okyere E, et al. Acceptability of rapid diagnostic test-based management of malaria among caregivers of under-five children in rural Ghana[J]. PLoS One,2012,7(9):e45556. [12] 王真瑜,张耀光,江莉,等. 上海市1例输血性三日疟病例的实验室检测分析与诊断[J]. 中国血吸虫病防治杂志,2015,27(4):362-366. [13] 高世同,张仁利,黄达娜,等. 基于SSUrRNA基因序列的间日疟原虫LAMP检测技术的建立[J]. 中国病原生物学杂志,2011,24(3):197-200. [14] 祝卫东. 干血滴中染色体外疟原虫DNA的PCR扩增及测序[J]. 国外医学(寄生虫病分册),1999,37(1):38-39. [15] 师永霞,黄吉城,苏锦坤,等. 广东口岸首例输入性卵形疟病例的诊断分析[J]. 中国寄生虫学与寄生虫病杂志,2014,32(2):156-158.
2016, Vol. 23 
