Abstract：Objective To compare the value of two nested-PCR methods in detecting Treponema pallidum (Tp) isolates in whole blood from patients with secondary syphilis. Methods Two nest-PCR methods targeted at Tp-tpp47 and Tp-polA were developed and used to amplify Treponema pallidum DNA in whole blood. Results Among 353 Tp seropositive whole blood samples, 142 (40.2%) and 132 (37.4%) samples were respectively positive for nPCR-tpp47 and nPCR-polA, indicating a statistically significant difference (P<0.05). The positive rates detected by the two nested-PCR methods in the group with high titers (RPR ≥ 1:8 or TPPA ≥ 1:2,560) were significantly higher than those in the group with low titers (RPR < 1:8 or TPPA < 1:2,560). Conclusions The nPCR-tpp47 is superior to the nPCR-polA in the detection of Treponema pallidum DNA in whole blood from patients with secondary syphilis, and it is a useful supplemental serological test for the diagnosis of syphilis.
肖勇健,刘卓然,李蓓,刘斌,彭菁. 两种巢氏PCR方法检测二期梅毒血液标本的比较[J]. 实用预防医学, 2016, 23(8): 904-906.
XIAO Yong-jian, LIU Zhuo-ran, LI Bei, LIU Bin, PENG Jing. Comparison of two nested-PCR methods for detection of Treponema pallidum in whole blood from patients with secondary syphilis. , 2016, 23(8): 904-906.
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2016, Vol. 23 
