Abstract：ObjectiveTo separated JE virus clinical strains, prepared high purity E protein antigen, and make colloidal gold for JE virus antibody detection.MethodsThe Japanese encephalitis virus E protein gene was amplified by RT-PCR and cloned into the prokaryotic expression vector to express the recombinant JEVE protein. The recombinant protein was purified by Ni column and refolded by dialysis bag.
陈沙彬,梅建华,雷永良,刘敏. 乙型脑炎病毒E结构域蛋白表达、纯化与胶体金的制备[J]. 实用预防医学, 2015, 22(12): 1445-1447.
CHEN Sha-bin, MEI Jian-hua, LEI Yong-liang, LIU Min. Expression, purification and preparation colloidal gold of Japanese encephalitis virus E structure protein. , 2015, 22(12): 1445-1447.
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