孕期亚临床甲减大鼠模型的建立

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摘要目的建立可靠的孕期亚临床甲减大鼠动物模型。方法将60只成年雌性Wistar大鼠随机分为三组：①完全甲减组：手术完全切除甲状腺;②亚临床甲减组：手术完全切除甲状腺后,予L-T₄微渗泵1.0µg/（100g·d）浓度持续皮下泵入,自怀孕第15天剂量改为1.05µg/（100g·d）;③正常对照组：行甲状腺假手术。各组大鼠在基础状态、孕前和怀孕后第10、13、17天和分娩当天采血测定甲状腺功能。结果各组大鼠在基础状态时,血清TSH和TT₄水平无统计学差异。完全甲减组大鼠在孕前和整个怀孕过程中及分娩当天血清TSH水平始终明显高于亚临床甲减组和正常对照组（都有P<0.01）,血清TT₄水平始终明显低于其他两组（都有P<0.01）。亚临床甲减组大鼠在孕前和整个怀孕过程中及分娩当天血清TSH水平始终明显高于正常对照组（都有P<0.05）,血清TT₄水平始终与正常对照组相当（都有P>0.05）。结论应用手术完全切除甲状腺,再予微渗泵皮下持续输入合适剂量的L-T₄可以成功建立孕期亚临床甲减大鼠模型。

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关键词 ：亚临床甲状腺功能减退症, 孕期, 大鼠模型, 微渗泵

Abstract：ObjectiveTo establish the animal model of pregnant rat with subclinical hypothyroidism. Methods60 female adult Wistar rat were divided into three groups: (i) overt hypothyroidism (OH) group: total removal of the rats’ thyroid. (ii) subclinical hypothyroidism (SCH) group: after total removal of the rats’ thyroid, levothyroxine were given to the rats by miniosmotic pumps and the rate was 1.0µg/(100g·d). (iii) control group: the false thyroid surgery was carried out. The serum samples were collected from all the three groups at baseline, preconception, 10^th, 13^th, 17^th gestational days and at delivery. Thyroid function was detected at all the time points. ResultsAt baseline, thyroid function indexes levels were similar among the three groups. After thyroid surgery, TSH levels of OH group were always higher than those of SCH group and control group (all P<0.01). And TT₄levels were always lower than those of other two groups (all P<0.01). In SCH group, from preconception to delivery serum TSH levels were always higher than those of control group (all P<0.05). Serum TT₄ levels were always similar with those of control group (all P>0.05). ConclusionAfter total removal thyroid and optimal dose of levothyroxine infusion with miniosmotic pump, a successful animal model of pregnant rat with subclinical hypothyroidism can be established.

Key words： Subclinical hypothyroidism Pregnancy Rat model Miniosmotic pump

R581.2

基金资助:高等学校博士学科点专项科研基金（项目编号：20092104120003）

通讯作者: 滕卫平 Email：twp@vip.163.com

作者简介: 于晓会（1978- ）,女,辽宁省瓦房店市人,博士研究生,副教授、副主任医师,研究方向是甲状腺疾病的流行病学和实验研究。

引用本文:

于晓会,刘爱华,朱帅俊,刘迪杰,单忠艳,滕卫平. 孕期亚临床甲减大鼠模型的建立[J]. 实用预防医学, 2015, 22(6): 686-688. YU Xiao-hui, LI Si-qi, ZHU Shuai-jun. Establishment of animal model of pregnant rat with subclinical hypothyroidism. , 2015, 22(6): 686-688.

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https://www.syyfyx.com/CN/ 或 https://www.syyfyx.com/CN/Y2015/V22/I6/686

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