Abstract：Objective To investigate the expressional alteration of COX2 in the activation of microglia BV2 cellsinduced by heavy metal manganese. Methods The BV2 cells in the experiments were divided into 3 groups, the control group, 50μM manganese exposure group and 100μMmanganese exposure group. The treatment time is 12h, 24h and 48h. Immunofluorescence assay was applied to show the activated BV2 cells that were induced by manganese exposure after 24h. Fluorescence real-time quantitative PCR (qRT-PCR)and Western Blot were performed to detect the quantitative alteration of COX2 in mRNA level and in protein level respectively. Results The morphological formof BV2 cells changed when treated by manganese after 24h. Cell bodies became larger and round while the processes became shorter or disappeared. Activated cells accounted for the partof total cells increased (P<0.05). The Results of qRT-PCR showed that the COX2 mRNA was upregulated after 12h and 24h manganese exposure (P<0.05). And the Results of Western Blot demonstrated that the COX2 protein was upregulated after 24h and 48h when treated by manganese (P<0.05). Conclusion The expression of molecule COX2 increased in BV2 cells when treated by manganese; and COX2 may play a role in the activation of mouse microglia cells induced by manganese exposure.
王晓静, 曹子鹏, 赵芳, 王迪雅, 苏鹏, 康蓓佩, 骆文静. 锰暴露诱导BV2小胶质细胞环氧合酶2表达变化[J]. 实用预防医学, 2016, 23(5): 535-538.
Wang Xiao-jing, Cao Zi-peng, Zhao Fang, Wang Di-ya, Su Peng, Kang Bei-pei, Luo Wen-jing. The expressionalalteration of cyclooxygenase2 induced by manganese exposurein microglia BV2 cells. , 2016, 23(5): 535-538.
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2016, Vol. 23 
