深圳市坪山新区白纹伊蚊对氯菊酯的抗性及羧酸酯酶活性研究

doi:10.3969/j.issn.1006-3110.2016.09.031

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摘要目的了解深圳市坪山新区白纹伊蚊野外种群对氯菊酯抗性及羧酸酯酶活性水平,为科学合理使用杀虫剂提供数据支持。方法采用幼虫浸渍法对白纹伊蚊进行氯菊酯抗药性测定,同时采用酶联免疫分析法对野外及敏感品系白纹伊蚊进行羧酸酯酶活性测试,分析比较野外品系和敏感品系羧酸酯酶活性的差异。结果坪山新区白纹伊蚊对氯菊酯的抗药性为4.17,属低抗性;野外品系与敏感品系白纹伊蚊羧酸酯酶活性浓度分别为(1 019.768±150.189)U/ml、(768.587±84.876)U/ml ,野外品系酶活性明显高于敏感品系,差异有统计学意义(t=3.256, P=0.012)。结论深圳市坪山新区白纹伊蚊对氯菊酯的低抗性可能与羧酸酯酶酶活性水平高有关。

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	吴能简
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关键词 ：白纹伊蚊, 氯菊酯, 羧酸酯酶, 抗药性

Abstract：Objective To investigate the resistance to permethrin and the activity level of carboxylesterase (CarE) to the wild strains in Aedes albopictus in Pingshan New District, Shenzhen City so as to provide data support for scientific and reasonable use of insecticides in vector control. Methods Larva immersion method was used to test the resistance of Aedes albopictus to permethrin. Enzyme-linked immunoassay was employed to detect the CarE activity of the wild strains and the sensitive strains in Aedes albopictus, and the differences in the CarE activity of the above-mentioned strains were analyzed and compared. Results Aedes albopictus from Pingshan District, Shenzhen City had low resistance to permethrin, with the resistance index of 4.17. The mean values of CarE in the wild and sensitive strains of Aedes albopictus were (1,019.768±150.189) U/ml and (768.587±84.876) U/ml respectively, with a statistically significant difference (t=3.256, P=0.012). Conclusions Aedes albopictus in Pingshan District, Shenzhen City has low resistance to permethrin, and it might be related to the increased CarE activity.

Key words： Aedes albopictus Permethrin Carboxylesterase Drug resistance

收稿日期: 2016-03-11

R384.1

引用本文:

吴能简, 陈伟文, 吴崧霖, 罗娘胡. 深圳市坪山新区白纹伊蚊对氯菊酯的抗性及羧酸酯酶活性研究[J]. 实用预防医学, 2016, 23(9): 1123-1125. WU Neng-jian, CHEN Wei-wen, WU Song-lin, LUO Niang-hu. Resistance to permethrin and activity of carboxylesterase in Aedes albopictus in Pingshan New District, Shenzhen City. , 2016, 23(9): 1123-1125.

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