Abstract：Objective To explore the subchronic oral toxicity of flutriafol TC, and to preliminarily analyze its effects on the blood system, liver and kidney functions of rats. Methods The experiment was performed according to the requirement of Toxicological Test methods of Pesticides for Registration (GB15670-1995). 80 SD rats of clean grade were randomly divided into four groups based on their body weight, with 10 male and 10 femele rats in each group. The rats in the three flutriafol TC treated groups were respectively fed with diet containing flutriafol TC 37.5, 150 and 600 mg/kg for 90 days. The clinical manifestations, food consumption and body weight of rats in each group were observed. Haematological parameters, clinical chemistry parameters, organ weight, organ coefficients and histopathological changes were examined at the end of the experiment. Results The actual doses of flutriafol TC taken by female rats in the three dose groups were 2.8, 12.2, and 47.2mg/kg respectively, and by male rats were 2.8, 11.3 and 47.1 mg/kg. During the period of the experiment, activity, food and water consumption of male and female rats in each dose group were normal, and no obvious toxicity symptoms appeared. Compared with the control group, WBC of male rats in the high-dose group decreased significantly. Blood urea nitrogen (BUN) and creatinine (CREA) of rats in the medium- and high-dose groups increased significantly. Total bilirubin (TBIL) of female rats in the high-dose group increased obviously. Aspartate amino-transferase (AST) and lactic dehydrogenase (LDH) of female rats in the medium- and high-dose groups increased significantly. No statistically significant difference or biological significance was found in organ weight and organ coefficients of male and females rats between each dose group and the control group (P>0.05). General anatomy indicated that no meaningful pathological changes induced by flutriafol TC were observed. Conclusions Long-term exposure to flutriafol TC has toxicity and potential pathogenic risk to the blood system, liver and kidney functions of rats. The maximum non-effect level of flutriafol TC is 2.8 mg/(kg bw·d) in female and male rats.
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2017, Vol. 24 
