Abstract：Objective To analyze the surveillance data about influenza and hemagglutinin (HA) gene of influenza A virus in Lishui City in 2017-2019 so as to understand the epidemic situation, subtype composition and gene phylogenetic characteristics of influenza A virus in Lishui City. Methods Data regarding influenza-like illness (ILI) cases and etiological detection results of influenza virus were collected from sentinel hospitals of influenza surveillance in Lishui City from 2017 to 2019, and then analyzed by descriptive epidemiological methods. The positive specimens detected by real-time fluorescence quantitative RT-PCR were isolated by MDCK cells, and the HA gene sequences of H1N1 and H3N2 influenza viruses were specifically amplified and analyzed by biological software. Results The clinic visit percentage of ILI cases in Lishui City in 2017-2019 was 5.70%. ILI% in different years showed statistically significant differences, and the overall positive rate was 17.08%. The positive detection rate of influenza virus displayed a gradually decreasing trend with the increase of age, but no statistically significant difference was found in the positive rate between different genders. New A (H1N1), seasonal H3N2, Yamagata and Victoria were all detected, and the distribution of each type in different years and different age groups had statistically significant differences. There was a positive correlation between ILI% and the positive detection rate of influenza virus (rs=0.576, P<0.05). The completed HA gene sequences with a length of 1,701 bp were obtained from 19 new A (H1N1) and 27 seasonal H3N2. H1N1 isolates all belonged to a single 6B.1 evolutionary branch in the post-pandemic period of 2009, with 14 amino acid substitutions. H3N2 isolates werecopolymerized in 3C.2a which was prevalent since 2015, and differentiated into 3C.2a1 and 3C.2a2, with 18 amino acid substitutions. Conclusion The epidemic trend of influenza in Lishui City from 2017 to 2019 was basically stable, which was in line with the epidemic characteristics of the southern region. There was no antigenic drift of H1N1 and H3N2 by molecularevolutionary analysis, which showed poor match with the 2018-2019 vaccine strains of the same period, but good match with the 2020-2021 updated vaccine strains.
叶碧峰, 王晓光, 叶灵, 陈秀英, 梅建华. 2017—2019年丽水市流感监测和甲型流感病毒HA基因进化分析[J]. 实用预防医学, 2022, 29(1): 1-5.
YE Bi-feng, WANG Xiao-guang, YE Ling, CHEN Xiu-ying, MEI Jian-hua. Surveillance on influenza and molecular evolutionary analysis of hemagglutinin gene of influenza A virus in Lishui City, 2017-2019. , 2022, 29(1): 1-5.
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