Abstract：Objective To investigate the situation of detecting the novel coronavirus 2019 (2019-nCoV) in different stages of coronavirus disease 2019 (COVID-19) and various specimens so as to provide a scientific basis for improving the protocol regarding COVID-19 diagnosis, treatment, prevention and control. Methods Anal, blood and pharyngeal swab specimens of 71 COVID-19 confirmed cases and 25 asymptomatic patients infected with 2019-nCoV in Loudi City were collected from February 26 to 27 in 2020. Real-time fluorescence reverse transcriptase polymerase chain-reaction was used to detect 2019-nCoV nucleic acid, and the detection results were analyzed. Results We collected 96 faeces specimens, 95 pharyngeal swab specimens and 83 blood specimens. There were statistically significant differences in the detection rate of 2019-nCoV nucleic acid in the anal swab specimens among different age groups. The detection rate of 2019-nCoV nucleic acid was higher in the asymptomatic infection patients than in the COVID-19 confirmed cases (47.06% vs. 12.70%) as well as higher in patients aged <40 years than in patients aged ≥40 years (37.5% vs. 8.33%). No statistically significant difference was found in the detection rate of 2019-nCoV nucleic acid in the pharyngeal swab specimens between the asymptomatic infection patients and the COVID-19 confirmed cases (12.5% vs. 11.3%). The detection rate of antibodies to 2019-nCoV in the blood specimens was 94.0%. Conclusions 2019-nCoV nucleic acid could be detected in the faeces and pharyngeal swab specimens of discharged patients, and the detection rate of 2019-nCoV nucleic acid in the faeces swab specimens was higher in the asymptomatic infection patients than in the COVID-2019 confirmed patients. The results suggest that special attention should be paid to the role of the asymptomatic infection patients in the spread of the epidemic. It is necessary to strengthen sustained follow-up and management of the asymptomatic infection patients and discharged patients, and perform further evaluation of the infectivity based on various indicators.
杨青廷, 刘敏, 杨纲, 刘建军, 朱攀, 申鸽, 梁琦, 龙思旋, 杨雪艳, 周丹, 戴苗, 吴锦如. 新型冠状病毒感染者核酸和抗体检测结果分析[J]. 实用预防医学, 2021, 28(2): 143-146.
YANG Qing-ting, LIU Min, YANG Gang, LIU Jian-jun, ZHU Pan, SHEN Ge, LIANG Qi, LONG Si-xuan, YANG Xue-yan, ZHOU Dan, DAI Miao, WU Jin-ru. Results of 2019-nCoV nucleic acid and antibody detection in patients with COVID-19. , 2021, 28(2): 143-146.
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2021, Vol. 28 
