敌敌畏对人神经母细胞瘤细胞SK-N-SH的氧化损伤作用

doi:10.3969/j.issn.1006-3110.2017.06.002

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摘要目的研究敌敌畏(O,O-dimethyl-O-2,2-dichlorovinylphosphate,DDVP)对人神经母细胞瘤细胞(SK-N-SH)的氧化损伤作用。方法 MEM培养基培养 SK-N-SH 细胞,细胞计数测定SK-N-SH细胞群体倍增时间;不同浓度(0~120 μmol/L)DDVP 作用SK细胞48 h后,MTT法测定SK-N-SH 细胞48 h半数抑制浓度(IC50);巴氏染色后观察细胞形态变化;同时分别检测细胞内超氧化物歧化酶(SOD)活性、过氧化氢酶(CAT)活性、丙二醛(MDA)含量的变化。结果SK-N-SH细胞群体倍增时间(36.28±1.46)h; 48 h半数抑制浓度(IC50)为(105.22±5.80)μmol/L ;随着 DDVP 染毒浓度的增高,细胞内 MDA 的含量逐渐增高,SOD、CAT活性逐渐降低,呈现明显的剂量—效应关系(P＜0.05)。结论DDVP 对 SK-N-SH 细胞有明显的氧化损伤作用。

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关键词 ： SK-N-SH细胞, 敌敌畏, 丙二醛, 超氧化物歧化酶, 过氧化氢酶, 氧化损伤

Abstract：Objective To study the oxidative damage of O,O-dimethyl-O-2,2-dichlorovinylphosphate (DDVP) to human neuroblastoma SK-N-SH cells. Methods SK-N-SH cells were cultured by minimum essential medium, and counted to determine the population doubling time. The SK-N-SH cells were treated with DDVP at different concentrations (0-120μmol/L) for 48 hours, and thenthe half maximal inhibitory concentration (IC₅₀) was measured by MTT assay. Cell morphological changes were observed under light microscopy by Giemsa staining; meanwhile, the activity of superoxide dismutase (SOD) and catalase (CAT) as well as intracellular malondialdehyde (MDA) content were detected. Results The population doubling time of SK-N-SH cells was (36.28±1.46) hours. The IC₅₀ of DDVP treatment for 48 hour in SK-N-SH cells was (105.22±5.80) μmol/L. With the increased concentration of DDVP, intracellular MDA content gradually increased, while the activity of SOD and CAT gradually decreased, showing a significant dose-response relationship (P<0.05). Conclusions DDVP can cause oxidative damage to human neuroblastoma SK-N-SH cells.

Key words： SK-N-SH cell O,O-dimethyl-O-2,2-dichlorovinylphosphate malondialdehyde superoxide dismutase catalase oxidative damage

收稿日期: 2016-11-17

R-33

基金资助:国家自然科学基金(81172712;81673227)

通讯作者: 龙鼎新(1972-),E-mail:dxlong99@163.com。

作者简介: 杨裔(1988-),男,湖南长沙人,硕士研究生,研究方向神经毒理。

引用本文:

杨裔, 朱家佳, 唐乖, 龙鼎新. 敌敌畏对人神经母细胞瘤细胞SK-N-SH的氧化损伤作用[J]. 实用预防医学, 2017, 24(6): 646-649. YANG Yi, ZHU Jia-jia, TANG Guai, LONG Ding-xin. Dichlorvos-induced oxidative damage in human neuroblastoma SK-N-SH cells. , 2017, 24(6): 646-649.

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