Abstract：Objective To explore the effect and mechanism of silencing galectin-3 gene expression by RNA interference on the proliferation and apoptosis of cervical carcinoma cell line. Methods The total protein in the cervical cancer tissues and the corresponding cancer-adjacent tissues was extracted, and then the expression of galectin-3 protein was detected by Western blot. Human cervical carcinoma HeLa cells were divided into the normal control group, the siRNA-control group and the siRNA-galectin-3 group, and the cells in each group were transfected for 48 hours, then the expression of galectin-3 protein was detected by Western blot. Cell proliferation and cell apoptosis were respectively detected by CCK8 assay and flow cytometry. The expression of ki67, proliferating cell nuclear antigen (PCNA), cleaved caspase3, β-catenin and cyclin D1 proteins were detected by Western blot. Results The expression of galectin-3 protein was significantly higher in the cervical carcinoma tissues than in the paracancerous tissues (P<0.01). siRNA-galectin-3 transfection could significantly reduce the expression of galectin-3 protein. The cell survival rate in the siRNA-galectin-3 group decreased significantly as compared with the normal control group and the siRNA-control group, while the cell apoptosis rate increased significantly. The number of positive cells of ki67 and PCNA and the expression of ki67, PCNA, β-catenin and cyclin D1 proteins in the siRNA-galectin-3 group significantly reduced as compared with the normal control group and the siRNA-control group, while the expression of cleaved caspase3 was significantly up-regulated (P<0.01). Conclusions Inhibition of galectin-3 gene expression by RNA interference can reduce the proliferation of cervical cancer cells and induce cell apoptosis, and the mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.
刘娟妮, 陈琳, 杨婷, 张艺博, 闫飞艳. RNAi抑制galectin-3基因表达对宫颈癌细胞增殖及凋亡的影响机制[J]. 实用预防医学, 2017, 24(12): 1525-1528.
LIU Juan-ni, CHEN Lin, YANG Ting, ZHANG Yi-bo, YAN Fei-yan. Effect and mechanism of silencing galectin-3 gene expression by RNA interference on the proliferation and apoptosis of cervical carcinoma cell line. , 2017, 24(12): 1525-1528.
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2017, Vol. 24 
