Abstract：Objective: To study the regulation of T-bet/GATA-3 and Th imbalance in asthmatic rats with Salvia and Dexamethasone. Methods: 80 SD rates were randomly divided into four groups: 1. Asthma model group;2.Normal control group; 3. Salvia group; 4.Combined group by Salvia and Dexamethasone.Each group has 20 rats. The airway responsiveness were observed; Lung tissue of SD rats were stained with HE, Pathologic examinations of lung tissue- stained were made. GATA-3 and T-bet expressions in lung tissue were detected by western-blots, respectively. Interleukin-4 (IL-4) and Interleukin-5（IL-5）in lung hemogenous were detected by ELISA. Results: The inflammation in slices of rat lung pathology in Salvia group and Combined group were mild compared with Asthma group, which most was in combined group. Airway resistance indicated that Asthma group>Salvia group>Combined group>Control group(P < 0.05), and histamine concentration had been increased with the airway resistance. Western-blots test the level of T-bet, which showed that Control group>Combined group>Salvia group>Asthma group(P < 0.01), however it contrasted in GATA-3 level. The level of IFN-γin lungs, which showed that Control group>Combined group>Salvia group>Asthma group(P < 0.01), however it contrasted in IL-4 level(r=0.893,P < 0.01). Conclusion Salvia injection may up regulate the expression of T-bet/GATA-3, and correcting Th1/Th2 imbalance, which play a synergistic role in anti-inflammation with Dexamethasone.
2015, Vol. 22 
