Abstract：ObjectiveTo establish a real time fluorescent quantitative RT-PCR assay for the simultaneous identification of human rhinovirus (HRV) so as to provide a rapid diagnosis method for the virus laboratory detection,epidemiological surveillance and emergency treatment. MethodsThe specific primers and probes were designed according to the target gene of 5'UTR of HRV. The specificity,sensitivity and repeatability of the real time RT-PCR assay in detecting HRV were analyzed.HRV were detected in 384 clinical respiratory infection samples by this newly establish method. Results The newly establish real time fluorescent quantitative RT-PCR shows strong specificity in identifying HRV, but not in HRV, H1N1, HCoV and influenza virus. The result of standard concentration HRV product tested by real time quantitative PCR was prove that the sensitivity of detecting HRV was 10 copies/µl. Ct average coefficient of variation was 0.58%~2.41% in the repeated test. The newly establish method was faster than the traditional method in detecting HRV of 384 clinical respiratory infection samples and total 21 positive strains of HRV were found. ConclusionThis real-time fluorescence RT-PCR assay can simultaneously,rapidly,sensitively and specifically detect HRV. It is capable of early diagnosis and molecular epidemiology investigation of HRV.
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2015, Vol. 22 
