Protective effect of genistein on osteoporosis induced by lead poisoning in SD rats
DONG Xin1, LIU Chun-yan2, SHEN Xiang-li3, ZHANG Jian-bin2
1. Tangdu Hospital, Fourth Military Medical University, Xi’an, Shaanxi 710038, China; 2. Department of Military Preventive Medicine, Fourth Military Medical University, Xi’an, Shaanxi 710032, China; 3. Shaanxi University of Traditional Chinese Medicine, Xi’an, Shaanxi 712046, China
Abstract:Objective To elucidate the effect of lead exposure on osteoporosis in rats through detecting femoral bone, biomechanical indexes and pathological bone histomorphometry indexes of rats with lead poisoning, to further study the feasibility of genistein as prevention and treatment medicine for osteoporosis induced by lead exposure in rats, and to provide laboratory evidence for effect study on lead poisoning and its prevention and treatment. Methods Rats were exposed to lead acetate in drinking water to establish lead poisoning models, and genistein was administered by gavage intervention. Atomic absorption spectrophotometer was used to determine lead content of femur in SD rats of each group. The bone mineral density (BMD) of rats was detected by X-ray absorptiometry. The INSTRON1195 electronic tensile test machine was employed for detecting biomechanical indexes of femoral bone of the rats, and then the effect of lead exposure on osteoporosis in the rats and the protective effect of genistein were explored. Results The values of femur dry weight, ash weight, bone calcium and BMD of lead-exposed rats decreased significantly as compared with the control group, but the values of femur dry weight and ash weight and BMD increased significantly (P<0.05) after administration of genistein. Lead-exposed rats’ indexes like femoral maximum loading(MaxL), maximum strain (MaxS), flexibility loading(FleL)and flexibility strain (FleS)significantly decreased, but the biomechanical indexes were improved significantly after the intervention with genistein, showing statistically significant differences (all P<0.05). Conclusions Chronic lead exposure can cause femur osteoporosis in rats. Genistein has some protective effects on lead-induced osteoporosis.
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