Abstract：With the rapid development of molecular diagnostic technology, TaqMan array card, as an emerging pathogen screening technology, has the characteristics of rapidness, high efficiency, sensitivity and accuracy, and its application research is increasing year by year, especially in the fields of disease surveillance, clinical diagnosis and treatment, and food hygiene. In this paper, we will review the developments in the application of TaqMan array card technology, aiming to provide new directions and new ideas for researchers.
[1] Manz A,Fettinger J,Verpoorte E,et al. Micromachining of monocrystalline silicon and glass for chemical analysis systems. A look into next century’s technology or just a fashionable craze?[J]. TrAC, Trends Anal Chem, 1991,10(5):144-149. [2] 林冬果, 林锦琼, 李佩文,等. 开放式微流控芯片上的肿瘤组织微阵列构建[J]. 分析化学, 2018, 46(1):113-120. [3] Park S, Zhang Y, Lin S, et al. Advances in microfluidic PCR for point-of-care infectious disease diagnostics[J]. Biotechnol Adv, 2011, 29(6):830-839. [4] 廖泽荣, 李永瑞, 古乐,等. 基于微流控芯片的细胞外囊泡分离技术研究进展[J]. 色谱, 2019, 37(4):343-347. [5] 姜浩, 吕雪飞, 赵可心. 基于微流控芯片的核酸适配体筛选技术研究进展[J]. 分析化学, 2020, 48(5):590-600. [6] 何浩延, 黄恩奇, 黎柱均,等. 微流控芯片液滴数字化分析用于快速定量检测细菌[J]. 分析化学, 2020, 48(7):855-862. [7] Steensels D,Reynders M,Descheemaeker P,et al. Clinical evaluation of a multi-parameter customized respiratory TaqMan array card compared to conventional methods in immunocompromised patients[J]. J Clin Virol, 2015, 72:36-41. [8] Rachwal PA, Rose HL, Cox V, et al. The potential of TaqMan array cards for detection of multiple biological agents by real-time PCR[J]. PLoS One, 2012, 7(4):e35971. [9] 王彦波, 荆红波, 李湛,等. Taqman低密度微流体芯片技术在呼吸道传染病病原体快速检测中的应用分析[J]. 中国卫生检验杂志, 2019, 29(6):641-643,657. [10] 徐君怡, 曹际娟, 郑秋月,等. 基于Taqman微流控芯片技术高通量检测17种转基因玉米品系[J]. 分析化学, 2020, 48(11):1477-1485. [11] 虞惠贞, 侯寒黎, 尹文秀,等. 利用TaqMan微流控阵列同步检测多种动物源性成分[J]. 浙江大学学报:农业与生命科学版, 2020, 46(2):189-200. [12] 王欣,虞典元,胡小平,等.2017—2019年孝感市儿童呼吸道病毒感染流行病学特征[J].实用预防医学, 2021, 28(9):1071-1075. [13] 潘明, 李天舒, 刘李,等. 成都市发热呼吸道症候群患者病毒感染调查[J]. 预防医学情报杂志, 2011, 27(11):861-864. [14] 蓝雨, 王大燕, 马学军,等. 呼吸道病毒实验室诊断的现状和进展[J]. 中华结核和呼吸杂志, 2014,37(1):6-8. [15] 刘可可. Taqman低密度微流体芯片技术在呼吸道病原体中的应用研究[D]. 北京: 中国人民解放军军事医学科学院, 2016. [16] Gaur B, Saha S, Iuliano AD, et al. Use of TaqMan array card for the detection of respiratory viral pathogens in children under 5 years old hospitalised with acute medical illness in Ballabgarh, Haryana, India [J]. Indian J Med Microbiol, 2019, 37( 1):105-108. [17] Kodani M, Yang G, Conklin LM, et al. Application of TaqMan low-density arrays for simultaneous detection of multiple respiratory pathogens[J]. J Clin Microbiol, 2011, 49(6):2175-2182. [18] Jones NK, Conway Morris A, Curran MD, et al. Evaluating the use of a 22-pathogen TaqMan array card for rapid diagnosis of respiratory pathogens in intensive care[J]. J Med Microbiol, 2020,69(7):971-978. [19] 陈峰, 吴海磊, 徐聪灵,等. 9种呼吸道病毒微流体芯片检测体系构建与应用[J]. 病毒学报, 2020, 36(4):624-633. [20] Waller JL,Diaz MH,Petrone BL,et al. Detection and characterization of Mycoplasma pneumoniae during an outbreak of respiratory illness at a university[J]. J Clin Microbiol, 2014, 52(3):849-853. [21] 刘师文, 熊英, 李健雄,等. 一起人腺病毒55型引起的儿童发热暴发疫情病原学鉴定[J]. 实验与检验医学, 2021, 39(3):738-741. [22] Ritter JM, Seixas JN, Walong E, et al. Histopathology is key to interpreting multiplex molecular test results from postmortem minimally invasive tissue samples[J]. Clin Infect Dis, 2021, 73(Suppl 5):S351-S359. [23] Roberts DJ, Njuguna HN, Fields B, et al. Comparison of minimally invasive tissue sampling with conventional autopsy to detect pulmonary pathology among respiratory deaths in a resource-limited setting[J]. Am J Clin Pathol, 2019, 152(1):36-49. [24] Boschi-Pinto C, Velebit L, Shibuya K. Estimating child mortality due to diarrhoea in developing countries[J]. Bull World Health Organ, 2008, 86(9):710-717. [25] Jones NK,Conway Morris A,Curran MD,et al. A laboratory-developed TaqMan array card for simultaneous detection of 19 enteropathogens[J]. J Clin Microbiol, 2013,51(2):472-480. [26] Zhou HL,Bessey T,Wang SM,et al. Burden and etiology of moderate and severe diarrhea in children less than 5 years of age living in north and south of China: prospective, population-based surveillance[J]. Gut Pathog, 2021, 13(1):33. [27] Lertsethtakarn P, Silapong S, Sakpaisal P, et al. Travelers' diarrhea in Thailand: a quantitative analysis using TaqMan (R) array card[J]. Clin Infect Dis, 2018,67(1):120-127. [28] Lappan R, Henry R, Chown SL, et al. Monitoring of diverse enteric pathogens across environmental and host reservoirs with TaqMan array cards and standard qPCR: a methodological comparison study[J]. Lancet Planet Health, 2021, 5(5):e297-e308. [29] Pedrosa de Macena LDG, Castiglia Feitosa R, Vieira CB, et al. Microbiological assessment of an urban lagoon system in the coastal zone of Rio de Janeiro, Brazil[J]. Environ Sci Pollut Res Int, 2021, 28(1):1170-1180. [30] 郭天宇. 开展21世纪海上丝绸之路沿线国家输入性病媒生物监测与防控研究,服务国家发展战略,切实维护国门安全[J]. 口岸卫生控制, 2020, 25(3):23-27. [31] 刘起勇. 新时代媒介生物传染病形势及防控对策[J]. 中国媒介生物学及控制杂志, 2019,30(1):1-6,11. [32] Amoako N, Duodu S, Dennis FE, et al. Detection of dengue virus among children with suspected malaria, Accra, Ghana[J]. Emerg Infect Dis, 2018, 24(8):1544-1547. [33] Ali MA, James OC, Mohamed AA, et al. Etiologic agents of fever of unknown origin among patients attending Mnazi Mmoja Hospital, Zanzibar[J]. J Commun Health, 2020, 45(5):1073-1080. [34] Onyango CO, Loparev V, Lidechi S, et al. Evaluation of a TaqMan array card for detection of central nervous system infections[J]. J Clin Microbiol, 2017, 55(7):2035-2044. [35] Liu J, Ochieng C, Wiersma S, et al. Development of a TaqMan array card for acute-febrile-illness outbreak investigation and surveillance of emerging pathogens, including Ebola virus[J]. J Clin Microbiol, 2016, 54(1):49-58. [36] Munyua P, Osoro E, Hunsperger E, et al. High incidence of human brucellosis in a rural pastoralist community in Kenya, 2015[J]. PLoS Negl Trop Dis, 2021, 15(2):e0009049. [37] Moore CC, Jacob ST, Banura P, et al. Etiology of sepsis in Uganda using a quantitative polymerase chain reaction-based TaqMan array card[J]. Clin Infect Dis, 2019,68(2):266-272. [38] Abade A, Eidex RB, Maro A, et al. Use of TaqMan array cards to screen outbreak specimens for causes of febrile illness in Tanzania[J]. Am J Trop Med Hyg, 2018, 98(6):1640-1642. [39] 王玫, 刘淑玲, 邹琦,等. 食品质量安全的现状及对策[J]. 科技经济市场, 2010,26(12):73-74. [40] 郑重,孙月琳,陈友霞,等.2019—2020年烟台市食源性疾病流行病学特征分析[J]. 实用预防医学, 2022,29(9):1031-1034. [41] 周前进. 对虾重要病原微生物等温扩增微流体芯片检测体系的研发[D]. 宁波: 宁波大学, 2018. [42] 陈炯, 屠丽红, 陈艳新,等. 微流体芯片技术检测食源性致病菌的应用研究[J]. 上海预防医学, 2014, 26(6):330-334. [43] Ambros V. The functions of animal microRNAs[J]. Nature, 2004,431(7006):350-355. [44] Alvarez-Garcia I, Miska EA. MicroRNA functions in animal development and human disease[J]. Development, 2005,132(21):4653-4662. [45] Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function[J]. Cell, 2004(116):281-297. [46] Stevic I, Müller V, Weber K, et al. Specific microRNA signatures in exosomes of triple-negative and HER2-positive breast cancer patients undergoing neoadjuvant therapy within the GeparSixto trial[J]. BMC Med, 2018, 16(1):179. [47] Pelloni M, Coltrinari G, Paoli D, et al. Differential expression of miRNAs in the seminal plasma and serum of testicular cancer patients[J]. Endocrine, 2017,57(3):518-527. [48] Rzepiel A, Kutszegi N, Gézsi A, et al. Circulating microRNAs as minimal residual disease biomarkers in childhood acute lymphoblastic leukemia[J]. J Transl Med, 2019, 17(1):372. [49] Gress TM, Lausser L, Schirra LR, et al. Combined microRNA and mRNA microfluidic TaqMan array cards for the diagnosis of malignancy of multiple types of pancreatico-biliary tumors in fine-needle aspiration material[J]. Oncotarget, 2017, 8(64):108223-108237. [50] Langmann T, Mauerer R, Schmitz G. Human ATP-binding cassette transporter TaqMan low-density array: analysis of macrophage differentiation and foam cell formation[J]. Clin Chem, 2006, 52(2):310-313. [51] Porter C, Constantin-Teodosiu D, Constantin D, et al. Muscle carnitine availability plays a central role in regulating fuel metabolism in the rodent[J]. J Physiol, 2017, 595(17):5765-5780. [52] Dalla Costa L, Emanuelli F, Trenti M, et al. Induction of terpene biosynthesis in berries of microvine transformed with VvDXS1 alleles[J]. Front Plant Sci, 2018, 8:2244. [53] Foongladda S,Banu S,Pholwat S,et al. Comparison of TaqMan® array card and MYCOTB(TM) with conventional phenotypic susceptibility testing in MDR-TB[J]. Int J Tuberc Lung Dis, 2016, 20(8):1105-1112. [54] Pholwat S,Liu J,Taniuchi M,et al. Genotypic antimicrobial resistance assays for use on E. coli isolates and stool specimens[J]. PLoS One, 2019, 14(5):e0216747. [55] Pholwat S, Liu J, Stroup S, et al. The malaria TaqMan array card includes 87 assays for plasmodium falciparum drug resistance, identification of species, and genotyping in a single reaction[J]. Antimicrob Agents Chemother, 2017, 61(5):e00110-e00117. [56] Zhao C, Wang X, Zhang C, et al. Development of a TaqMan array card to target 21 purulent meningitis-related pathogens[J]. BMC Infect Dis, 2019, 19(1):289. [57] Zhang C,Zheng X,Zhao C,et al. Detection of pathogenic microorganisms from bloodstream infection specimens using TaqMan array card technology[J]. Sci Rep, 2018, 8(1):12828. [58] Kodani M,Mixson-Hayden T,Drobeniuc J, et al.Rapid and sensitive approach to simultaneous detection of genomes of hepatitis A, B, C, D and E viruses[J]. J Clin Virol, 2014, 61(2):260-264. [59] Velaphi SC, Westercamp M, Moleleki M, et al. Surveillance for incidence and etiology of early-onset neonatal sepsis in Soweto, South Africa[J]. PLoS One, 2019, 14(4):e0214077. [60] Diaz MH,Waller JL,Theodore MJ,et al. Development and implementation of multiplex TaqMan array cards for specimen testing at child health and mortality prevention surveillance site laboratories[J]. Clin Infect Dis, 2019, 69(Suppl 4):S311-S321.
2023, Vol. 30 
