Abstract：Objective To explore the application of matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) in homology analysis of Pseudomonas putida in neonatal bloodstream infection. Methods Seven strains of Pseudomonas putida in neonatal bloodstream infection were collected from neonatal ward of Hunan Children's Hospital. VITEK-compact 2 was used to detect drug sensitivity. MALDI-TOF MS and SARAMIS software were employed to detect and analyze the homology of the Pseudomonas putida strains. Meanwhile, whole genome sequencing (WGS) was applied to verifying the analysis results of MALDI-TOF MS. Results The 7 Pseudomonas putida strains were not sensitive to third- and fourth-generation cephalosporins and carbapenase antibiotics, but sensitive to amikacin and levofloxacin. By using MALDI-TOF MS and SARAMIS software, it was found that the relative similarity in the 7 Pseudomonas putida strains ranged from 80% to 90%. WGS analysis revealed that all the 7 Pseudomonas putida strains were MLST-128, with an average nucleotide identity (ANI) being >99.9%. Conclusion The 7 Pseudomonas putida strains in this study had similar drug resistance phenotypes. MALDI-TOF MS found that they were of the same type with the relative similarity being 80%-90%. WGS confirmedthat they were from the same strain. MALDI-TOF MS can be used for preliminary and rapid homology analysis of Pseudomonas putida.
刘健龙, 李先斌, 刘灿, 胡琼, 莫丽亚. MALDI-TOF MS在新生儿血流感染恶臭假单胞菌同源性分析中的应用[J]. 实用预防医学, 2022, 29(12): 1484-1487.
LIU Jian-long, LI Xian-bin, LIU Can, HU Qiong, MO Li-ya. Application of MALDI-TOF MS to homology analysis of Pseudomonas putida in neonatal bloodstream infection. , 2022, 29(12): 1484-1487.
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2022, Vol. 29 
