Abstract:Objective:To explore the effect of preincubation conditions on the time to detection (TTD) of blood culture of common blood infectious bacteria and provide evidence for shipping and handling the clinical blood culture specimens reasonably. Methods In order to mimic blood infection specimens, Staphylococcus aureus (S. aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa(P. aeruginosa) at 10 and 100 CFU/ml were inoculated into standard aerobic or anaerobic blood culture bottles, and then were preincubated at 25°C or 37°C for 0, 2, 4, 8, 12 or 24h. TTD was monitored using BacT/Alert 3D system. ResultsCompared with the groups without preincubation, TTD was significantly shortened following preincubation for 8 hr at 25°C and 4 hr at 37°C for S. aureus and E. coli, as well as for 12 hr at 25°C and 8 hr at 37°C for P. aeruginosa (P<0.05). No statistical differences in TTD were observed between different bacterial concentrations (P>0.05), or between aerobic and anaerobic incubation groups(P>0.05). The BacT/Alert 3D system reported false-negative results for P. aeruginosa following preincubation for 24 hr at 25°C or 37°C. ConclusionsTTD for the Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa in blood specimens is mainly affected by temperature and duration of preincubation rather than by input amount of CFU or type of blood culture bottle. So blood specimens that can’t be put in BacT/Alert 3D system in time should be preincubated at 37°C, but for no more than 24h.
谭黎明,谢良伊,程振波,李建英,毛玉环. 预培养对血液常见感染菌血培养阳性报警时间的影响[J]. 实用预防医学, 2015, 22(4): 416-419.
TAN Li-ming, XIE Liang-yi, CHENG Zhen-bo, LI Jian-ying, MAO Yu-huan. Effect of preincubation on time to detection of blood culture of common blood infectious bacteria. , 2015, 22(4): 416-419.
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