Abstract:ObjectiveThe aim of this study was to investigate the combined effects of veliparib and doxorubicin on the proliferation and apoptosis of human hepatocellular carcinoma cell line BEL-7404. MethodsBEL-7404 cells were taken as the object of study and conventional culture was performed. The cells were treated with doxorubicin and/or veliparib for 24h. Cell proliferation were detected by MTT assay. DNA damage and cell apoptosis were measured by comet assay and flow cytometry using annexin V-FITC/PI staining respectively. The expression of cytochrome c in the mitochondrion and cytoplasm was detected by Western blotting. ResultsCompared with the control group and doxorubicin-treated group, the cell proliferation rate in the combined-treatment group was significantly lower(P<0.01), while the cell apoptosis rate was significantly higher(P<0.05). Furthermore, the DNA damage in the combined-treatment group was more serious and the expression of cytochrome c in cytoplasm was up-regulated(P<0.01).ConclusionsCombined treatment of veliparib and doxorubicin can inhibit proliferation and induce DNA damage and apoptosis in BEL-7404 cells. The mechanism probably involves increased intracellular cytochrome c expression.
李红娟,姜英,苏君梅,葛卫红. 维利帕尼和多柔比星联合作用对人肝癌耐药细胞株BEL-7404增殖和凋亡的影响[J]. 实用预防医学, 2015, 22(4): 398-402.
LI Hong-juan, JIANG Ying, SU Jun-mei, GE Wei-hong. Combined effects of veliparib and doxorubicin on the proliferation and apoptosis of human hepatocellular carcinoma cell line BEL-7404. , 2015, 22(4): 398-402.
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