Abstract：Objective To detect the contaminationby Legionella pneumophila in environmental water source of Shenzhen by conventional culture and nested PCR. Methods Cooling water, shower warm water, tap water and landscape water from stores, hotels and hospitals were collected during September, 2012 and November, 2012. Leginella pneumophilia was tested by conventional culture and nested PCR . Results Totally, 171 samples were collected. Legionella pneumophila was found in 64 samples by conventional culture and in 130 samples by nested PCR. The positive rates of Legionella pneumophila by the two methods were 37.4% and 76.0%, respectively. Conclusions Legionella pneumophilia exists in environmental water source of Shenzhen. Detection rate by nested PCR is higher than that by conventional culture.
2015, Vol. 22 
