Abstract：Objective To investigate the Contamination status of legionella in Hospital water environment and traceability investigation of legionella infection in hospital pneumonia cases. Methods 24 Cooling tower water and pipe network peripheral water samples of hospital were collected. The water samples were cultured for legionella. To know the situation and types of legionella infection,we survey 90 hospital pneumonia cases. Implementation of the questionnaire,deep sputum cultures and fluorescence quantitative PCR detection of legionella , urinary Antigen and serum antibody detection of legionella.To learn the source of legionella infection,the legionella strains which isolated from environment and cases were tested by the way of PFGE and SBT. Results The legionella positive rate of Cooling tower water was 100%(5/5), and 2 samples' legionella count were greater than 103cfu/L. The legionella positive rate of pipe network peripheral water samples of hospital was 21.1%(4/19), and 3 samples' legionella count were more than 103cfu/L.The average of residual chlorine in water was 0.03 mg/L.Legionella pollution serotypes were LP1, LP6. 15.6% (14/90) hospital pneumonia cases were infected by legionella. One case was positive by sputum culturing and PCR testing. 13 cases were positive by urinary antigen testing.16.4%(11/67)was CALs, 20.0%(3/15)was HALs.The strains separated from environment and patients,which spectrum and PFGE serotype were inconsistent. So, the source of legionella infection was unclear.Conclusion The investigated hospital have high levels contamination and high concentration of legionella, have lower residual chlorine concentration than the standard, which would likely to acquire legionellosis. We suggest regular monitoring legionella in hospital water environment, conduct Legionella disease related detection for hospital pneumonia cases, and disinfection of the water systems should be considered.
张琦, 陈茸, 张稷, 严洁, 张旭辉, 周伟杰. 医院水环境军团菌污染及住院肺炎病例军团菌感染来源调查[J]. 实用预防医学, 2016, 23(2): 147-150.
ZHANG Qi, CHEN Rong, YAN Jie, ZHANG Ji, ZHANG Xu-hui, ZHOU Wei-jie. Contamination status of legionella in Hospital water environment and traceability investigation of legionella infection in hospital pneumonia cases. , 2016, 23(2): 147-150.
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