Abstract：Objective:To explore the value of early diagnosis of NGAL and KIM-1 to AKI by studying the expression of them in rat model of AKI induced by gentamtcin sulfate. Methods:40 SPF male Sprague-Dawley rats randomly assigned to five groups.Four group rats of them were injected introperitoneally with gentamtcin sulfate by 100mg/kg,the control group were injected with saline at the same dosage.One by one group of rats were necropsied respectively on day 3,5,7 and 10 of drugs for renal pathological section,serum and urine specimen to detect the change of biochemical index and NGAL,KIM-1.The control group were necropsied two rats each time. Results:serum creatinine and blood urea nitrogen increased to（90.4±1.9）μmol/mL、（62.2±1.2）μmol/mL after 7 days; cystatinC increased to （0.24±0.03）μmol/mL after 5 days; the 24 hours microalbuminuria in urine increased to（38.43±1.74）mg/L after 8 days;pathological observation show renal tubular ectasia, the exfoliation and disappearance of renal tubular wall cells and the inflammatory cell infiltration wew occurred after 5 days; the NGAL in blood and NGAL,KIM-1 in urine respectively increased to（28.8±3.17）μg/mL、（3.63±0.50）μg/mL and（5.43±1.25）μg/mL after only 3 days, the KIM-1 in blood increased to（4.51±0.41）μg/mL after 5 days. Conclusion：The NGAL,KIM-1 in blood and urine can be regarded as a reliable index of drug preclinical evaluation and the early diagnosis of AKI with the same mechanism.
曾凡智, 肖创清. 硫酸庆大霉素诱导大鼠急性肾损伤模型血和尿NGAL,KIM-1的变化及意义[J]. 实用预防医学, 2016, 23(2): 140-143.
ZENG Fan-zhi, *XIAO Chuang-qing. The changes and significance of NGAL,KIM-1 in blood and urine in rat model of AKI induced by gentamycin sulfate. , 2016, 23(2): 140-143.
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2016, Vol. 23 
