Abstract：Objective To establish the high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) with isotope dilution for the determination of vomitoxin and its derivants in cereal grains. Methods The samples were extracted with acetonitrile-H2O(86+14,v/v),purified with HLB column, separated on a C18, and then analyzed with HPLC-MS under the negative ion multiple reaction monitoring mode. Results The linearities of DON and 3-ADON were in the concentration range of 2.0μg/L~500 μg/L, of 15-ADON in the concentration range of 10 μg/L~1000 μg/L, with the correlation coefficients higher than 0.9990. The limits of detection and the limits of quantitation were 0.10 μg/kg~1.6 μg/kg and 0.35 μg/kg~6.0 μg/kg, respectively. The average spiked recoveries ranged from 80.3% to 115.4%, and the relative standard deviation(RSD, n=6) ranged from 5.1% to 14.6%. Conclusions The high performance liquid chromatography-mass spectrometry( HPLC - MS/MS) with isotope dilution is easy, rapid and accurate. It can meet the requirements for the determination of the vomitoxin and its derivants in cereal grains.
周鸿艳, 黄方取, 刘洋. 高效液相色谱-串联质谱法测定谷物中呕吐毒素及其衍生物[J]. 实用预防医学, 2016, 23(10): 1269-1271.
ZHOU Hong-yan, HUANG Fang-qu, LIU Yang. Determination of vomitoxin and its derivants in cereal grains by high performance liquid chromatography-mass spectrometry. , 2016, 23(10): 1269-1271.
2016, Vol. 23 
