Abstract：Objective To explore the effects of arsenic exposure and estrogen receptor inhibitor on estrogen and its receptor and thyroid hormone receptor in rats, and to explain the pathway of thyroid toxicity of arsenic. Methods Rats drank water with sodium arsenite, and then were given estrogen receptor inhibitor (ICI182780) intervention. The levels of serum estradiol (E2), total triiodothyronine (TT3), total thyroxine (TT4) and thyroid stimulating hormone (TSH) in rats were detected by enzyme linked immunosorbent assay. Transmission electron microscope was used to observe thyroid histopathological structure changes in rats. Quantitative real-time polymerase chain reaction was employed to detect relative expression of thyroid tissue oestrogen receptor α (ERα) and thyroid hormone receptor α (TRα).TRα protein level in thyroid tissue was detected by western blot. Results After arsenic exposure, the level of E2 expression in female rats increased compared with the control group (P<0.05), but the relative expression of ERα and TRα in male and female rats of the low-arsenic group decreased compared with the control group (P<0.05). After high-dose arsenic exposure, the levels of TSH, TT3 and TRα expression in female rats increased compared with the control group, but the level of TT4 expression decreased compared with the control group (P<0.05). ICI182780 could reduce the increase in E2 expression level in female rats and also had a reverse regulation for changes in TT4, TSH, ERα and TRα expression in arsenic-exposed rats (P<0.05). Conclusion Low-arsenic exposure has a significant estrogen effect on female rats, and high-arsenic exposure can cause thyroid dysfunction or damage in rats. ERα may be one of the main ways for arsenic exposure to exert estrogen effects in the body. ICI182780 can antagonize estrogen effects caused by arsenic exposure and relieve damage to thyroid function.
李宏昀, 马晓薇, 江志红, 买哈巴·木合塔尔, 吴军. 大鼠砷暴露致雌激素效应及对甲状腺功能的影响[J]. 实用预防医学, 2021, 28(12): 1415-1420.
LI Hong-yun, MA Xiao-wei, JIANG Zhi-hong, MAIHABA Muhetaer, WU Jun. Estrogenic effect of arsenic exposure and its effect on thyroid function in rats. , 2021, 28(12): 1415-1420.
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