Abstract：Objective To monitor the contents of β-receptor agonists in livestock-meat marketed in Jinan city, and to provide a basis for livestock-meat industry regulation and the exposure risk assessment thereof. Methods Livestock-meat and offal were collected from different market sources in Jinan city from 2014 to 2019. Solid-phase extraction coupled with high-performance liquid chromatography tandem mass spectrometry was used to detect the contents of clenbuterol, ractopamine, salbutamol and terbutaline, and then their different residual levels were analyzed. Results A total of 397 livestock-meat samples were tested in the six years, and the detection rate of β-receptor agonist was 20.15%(80/397), of which the detection rate of clenbuterol was The detection rates of β-receptor agonists were significantly higher in livestock-meat samples collected from farmers’ markets than in ones collected from supermarkets(P<0.01), higher in beef and mutton samples than in pork samples(P<0.05), and higher in offal samples than in muscle samples(P<0.05). Conclusions The detection rate of clenbuterol is higher in livestock-meat marketed in Jinan city. Supervision of cattle and sheep breeding, slaughter and sales needs to be strengthened. There is a higher risk of exposure to β-residual agonists in beef and mutton, especially in viscera.
李士凯, 孙婷, 张鑫, 孟中华, 刘仲. 2014—2019年济南市市售畜肉中β-受体激动剂含量调查[J]. 实用预防医学, 2020, 27(8): 943-946.
LI Shi-kai, SUN Ting, ZHANG Xin, MENG Zhong-hua, LIU Zhong. Survey on contents of β-receptor agonists in livestock-meat marketed in Jinan city, 2014-2019. , 2020, 27(8): 943-946.
[1] Kern C, Meyer T, Droux S, et al. Synthesis and pharmacological characterization of beta 2-adrenergic agonist enantiomers: zilpaterol [J]. J Med Chem, 2009, 52(6):1773-1777. [2] Downie D, Delday MI, Maltin CA, et al. Clenbuterol increases muscle fiber size and GATA-2 protein in rat skeletal muscle in utero[J]. Mol Reprod Dev, 2008, 75(5):785-794. [3] Strydom PE, Frylinck L, Montgomery JL, et al. The comparison of three β-agonists for growth performance, carcass characteristics and meat quality of feedlot cattle[J]. Meat Sci, 2009, 81(3):557-564. [4] 蒋志伟,仕金,张雨梅,等. 莱克多巴胺对鼠的毒性研究[J]. 兽药与饲料添加剂, 2001, 6(6):4-6. [5] 马金魁.盐酸克伦特罗对成年大鼠睾丸类固醇激素合成急性调节蛋白(StAR)基因mRNA 表达的影响[D]. 广州:暨南大学,2009. [6] 任欣.莱克多巴胺对成年大鼠睾丸类固醇激素合成急性调节蛋白(StAR)基因mRNA 表达的影响[D]. 广州:暨南大学,2009. [7] Currie GP, Lee DK, Lipworth BJ. Long-acting beta2-agonists in asthma: not so smart[J]. Drug Saf, 2006, 29(8):647-656. [8] Pulce C, Lamaison D, Keck G, et al. Collective human food poisonings by clenbuterol residues in veal liver[J]. Vet Hum Toxicol, 1991, 33(5):480-481. [9] Suzuki J, Gao M, koyama T. Effects of the beta 2-adrenergic agonist clenbuterol on capillary geometry in cardiac and skeletal muscles in young and middle-aged rats[J]. Acta Physiol Scand, 1997, 161(3):317-326. [10] Ma JK, Zhu WJ. Effects of the β2-adrenoceptor agonist clenbuterol on testicular steroidogenic acute regulatory protein mRNA expression in adult rats[J]. J Vet Pharmacol Ther, 2010, 33(10):558-563. [11] Expert Committee on Food Additives. Evaluation of certain veterinary drug residues in food. Forty-seventh report of t he Joint FAO WHO[R]. Geneva: WHO, 1998:6-12. [12] 徐伟,程凤科,耿云,等. 浅谈“瘦肉精”检测方法及其应用[J]. 安徽农学通报,2012, 18(15):160-161. [13] 孙莉.瘦肉精在肉类食品中的残留问题及检测[J].食品安全导刊,2016(21):15. [14] 仓公敖,李莉,陈蓓,等.动物性食品中抗生素及瘦肉精残留量基线调查研究[J].中国卫生检验杂志,2003,13(4):422-423. [15] 梁照升,张正尧,鹿尘,等.周口市肉与肉制品中盐酸克伦特罗残留量调查分析[J].中国卫生检验杂志,2005,15(9):1110-1111. [16] 薛颖,吴国华,孟娟,等.北京市2002年生猪产品中克伦特罗污染状况调查[J].中华流行病学杂志,2003,24(8):654-656. [17] 何洁仪,马林,李迎月,等.广州市17起盐酸克伦特罗食物中毒分析及预防措施[J].中国食品卫生杂志, 2003,15(1):54-55. [18] 顾振华,郑雷军.上海盐酸克伦特罗食物中毒事件的分析与思考[J].中国食品卫生杂志, 2007,19(1):10-12. [19] 刘如春,张锡兴,陈田木,等.一起盐酸克伦特罗导致食物中毒事件调查[J].实用预防医学, 2013,20(9):1094-1096,1091. [20] 陆昱养,周伦敏,何云亚,等.一起瘦肉精引起的食物中毒调查报告[J].浙江预防医学, 2012,24(4):58,67.
2020, Vol. 27 
