Abstract：Objective To understand the infection and carrying status of Epstein-Barr virus (EBV) in healthy children in Chenzhou region, and to provide a scientific basis for correct diagnosis of EBV-related diseases and scientific prevention and treatment of EBV infection in children. Methods According to age, 350 healthy children (aged from 4 months to 14 years) selected from counties and districts of Chenzhou were divided into infant group (< 1 year old), early childhood group (1 year old-), preschool group (3 years old-), school age group (6 years old-) and elder children group (10-14 years old). EBV CA-IgG in serum was detect by immunological method, while EBV-DNA in peripheral blood mononuclear cell (PBMC) by real-time fluorescence quantitative PCR assay. The results were statistically analyzed. Results Among the 350 healthy children, the total positive rates of EBV CA-IgG and EBV-DNA were 67.14% and 50.86%, respectively, and the former was significantly higher than the latter (P=0.000). The positive rates of EBV CA-IgG and EBV-DNA and EBV-DNA viral load of nucleic acid positive patients showed no significant difference between boys and girls (all P>0.05). The positive rates of EBV CA-IgG and EBV-DNA and EBV-DNA viral load of nucleic acid positive patients showed statistically significant differencesamong children with different ages(all P<0.05). The positive rate of EBV CA-IgG increased with the increasing age. The positive rate of EBV-DNA increased firstly and then decreased with the increasing age. EBV-DNA viral load of nucleic acid positive patients decreased gradually with the increasing age. Same group comparison indicated that the positive rates of EBV CA-IgG and EBV-DNA in infant group, early childhood group and preschool group showed no statistically significant differences (all P>0.05), but those in school age group and elder children group showed statistically significant differences (all P<0.05). Comparison of different groups revealed that EBV-DNA viral load of nucleic acid positive patients was significantly higher in infant group and early childhood group than in other groups (P<0.05). Conclusions In the diagnosis of EBV infection, detection of EBV-DNA in PBMC can more reflect the actual infection level of EBV as compared with detection of EBV CA-IgG in serum, and the clinical value is high. The detection rate of EBV-DNAin healthy children is high, and there is widespread infection in vivo, but the viral load is not high and varies with the age. Therefore, we should make a comprehensive analysis and judgment according to the age of children and fully consider the presence of EBV carrying infection in prevention, diagnosis and treatment of EBV infection.
江杨华, 宋然, 刘巧突, 林应标. 郴州地区350例健康儿童EBV感染情况分析[J]. 实用预防医学, 2020, 27(6): 689-691.
JIANG Yang-hua, SONG Ran, LIU Qiao-tu, LIN Ying-biao. Status of Epstein-Barr virus infection in 350 healthy children in Chenzhou region. , 2020, 27(6): 689-691.
2020, Vol. 27 
