Abstract：Objective To analyze the correlation between the mutation of S protein sequence of hepatitis B virus (HBV) and the coexistence of HBsAg and anti-HBs in patients with chronic hepatitis B (CHB). Methods Thirty-three CHB patients with coexisting HBsAg and anti-HBs were randomly selected as the experimental group, and 33 CHB patients with HBsAg positive but anti-HBs negative as the control group. The S gene of HBV was amplified and sequenced. Results HBV in the two groups was B or C genotype, without other genotypes. No statistically significant differences were observed in age, gender, HBV genotype and serum HBV DNA level between the two groups (all P>0.05). The amino acid mutation rates in the N-terminal (2.61% vs. 1.21%), major hydrophilic region (1.69% vs. 0.48%), especially "α" determinant (3.23% vs. 0.63%) of S protein were significantly higher in the experimental group than in the control group (all P<0.01), but no statistically significant difference was found in the amino acid mutation rate in the C-terminal of S protein (1.75% vs. 1.22%, P>0.05). The most frequent amino acid mutation in the experimental group was located at position 126 of S protein. There were no statistically significant differences in the deletion or insertion mutations between the two groups (P>0.05). Conclusions The amino acid mutations, especially those in the "α" determinant, in the patients with CHB may be associated with the coexistence of HBsAg and anti-HBs.
申春梅, 唐思叶, 王妹芳, 贺乐奇. HBsAg与anti-HBs共阳性慢性乙肝患者病毒S蛋白序列分析[J]. 实用预防医学, 2018, 25(12): 1458-1461.
SHEN Chun-mei, TANG Si-ye, WANG Mei-fang, HE Le-qi. Sequence of S protein in chronic hepatitis B patients with coexisting HBsAg and anti-HBs. , 2018, 25(12): 1458-1461.
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2018, Vol. 25 
