Abstract:Fleas are not only important medical insects, but important propagation media of plague which is legal category A infectious disease in China. There are more than 2,500 species of fleas in the world, and they are rich in genetic diversity. Genetic diversity can not only reveal the genetic variation and system evolution and clear some problems of morphological classification, but provide an important scientific basis for the control and prevention of insect-borne infectious diseases as well. This paper reviews the application of molecular biology technologies (RAPD, SSR, SNP, GWAS, rDNA and mtDNA) to research on the genetic diversity of fleas.
[1] 吴厚永, 刘泉, 鲁亮, 等. 中国动物志昆虫纲蚤目[M].第2版. 北京:科学出版社, 2007:1-2174. [2] 解宝琦, 曾静凡. 云南蚤类志[M]. 昆明:云南科技出版社, 2001:1-456. [3] Hebert PDN, Cywinska A, Ball SL, et al. Biological identifications through DNA barcodes[J]. Proc R Soc Lond B Biol Sci, 2003, 270(1):313-321. [4] 吴云良, 包文斌, 张红霞, 等. 微卫星技术分析东北虎遗传多样性及亲缘关系[J]. 扬州大学学报, 2011, 32(1):87-91. [5] 胡艳红,迟德富. PAPD技术在昆虫学研究中的进展[J]. 应用生态学报, 2004, 15(8):1481-1486. [6] 曲娟娟, 于敏, 董蕾, 等. SNP在动植物遗传育种中的应用[J]. 东北农业大学学报, 2011, 42(1):9-13. [7] 陈珊珊, 周明芹. 浅析遗传多样性的研究方法[J]. 长江大学学报, 2010, 7(3):54-57. [8] 殷方媛, 李法财, 赵俊龙, 等. 动物寄生线虫遗传多样性的研究进展[J]. 中国寄生虫学与寄生虫病杂志, 2015, 33(5):387-392. [9] 钟敏, 蔺应学, 王英. 分子生物学技术在按蚊近缘种鉴定的应用进展[J]. 中国热带医学, 2014, 14(12):1529-1532. [10] Vobis M, Haese JD, Mehlhorn H, et al. Molecular phylogeny of isolates of Ctenocephalides felis and related species based on analysis of ITS1, ITS2 and mitochondrial 16S rDNA sequences and random binding primers[J]. Parasitol Res, 2004, 94(3):219-226. [11] 鲁亮, 张金桐, 吴厚永. 三种新蚤RAPD关系的初步研究[J]. 寄生虫与医学昆虫学报, 1999, 6(4):234-238. [12] Selkoe KA, Toonen RJ. Microsatellites for ecologists:a practical guide to using and evaluating microsatellite markers[J]. Ecol Lett, 2006, 9(5):615-629. [13] 国伟, 沈佐锐. 微卫星DNA标记技术及其在昆虫学上的应用[J]. 生物技术, 2004, 14(1):60-61. [14] Husseneder C, Garner SP, Foil LD, et al. Development of microsatellites for genetic analyses and population assignment of the cat flea (Siphonaptera:Pulicidae)[J].J Med Entomol, 2010, 47(6):1028-1033. [15] 唐立群, 肖层林, 王伟平. SNP分子标记的研究及其应用进展[J]. 中国农学通报, 2012,28(12):154-158. [16] 张雁明, 邢国芳, 刘美桃, 等. 全基因组关联分析:基因组学研究的机遇与挑战[J]. 生物技术通报, 2013, 33(1):1-6. [17] Bass C, Schroeder I, Turberg A, et al. Identification of the Rdl mutation in laboratory and field strains of the cat flea, Ctenocephalides felis (Siphonaptera:Pulicidae)[J]. Pest Manag Sci, 2004, 60(12):1157-1162. [18] Brunet S, Le Meter C, Murray M, et al. Rdl gene polymorphism and sequence leanalysis and relation to in vivo fipronil susceptibility instrains of the cat flea[J]. J Econ Entomol, 2009, 102(1):366-372. [19] 江世宏, 孟子烨, 陈晓琴, 等. 核糖体DNA序列分析在昆虫系统学研究中的应用[J]. 昆虫分类学报, 2008, 30(3):225-238. [20] 马婷婷, 陈光, 刘春香. ITS序列的特点及其在昆虫学研究中的应用[J]. 应用昆虫学报, 2011, 48(3):710-715. [21] Luchetti A, Trentini M, Pampiglione S, et al. Genetic variability of Tunga penetrans (Siphonaptera, Tungidae) sand fleas across South America and Africa[J]. Parasitol Res, 2007, 100(3):593-598. [22] Marrugal A, Callejón R, De RM, et al. Morphological, biometrical, and molecular characterization of Ctenocephalides felis and Ctenocephalides canis isolated from dogs from different geographical regions[J]. Parasitol Res, 2013, 112(6):2289-2298. [23] 鲁亮, 吴厚永. 新蚤属9种新蚤核糖体DNA ITS2序列变化[J]. 寄生虫与医学昆虫学报, 2002, 9(2):106-113. [24] 赵文静, 漆一鸣. 方形黄鼠蚤(Citellophilus tesquorum)二亚种 rDNA ITS1、 ITS2 序列的测定与分析[J]. 贵阳医学院学报, 2010, 35(3):240-242. [25] 刘会, 李正西, 李淑娟, 等. 线粒体 DNA 序列在半翅目异翅亚目昆虫分子系统学上的应用[J]. 昆虫分类学报, 2007, 29(4):265-274. [26] 郭仲龙, 袁明龙. 半翅目昆虫线粒体基因组学研究进展[J]. 中国科学:生命科学, 2016, 46(2):151-166. [27] Vobis M, D’ Haese J, Mehlhorn H, et al. Molecular biological investigations of Brazilian Tunga, isolates from man, dogs, cats, pigs and rats [J]. Parasitol Res, 2005, 96(2):107-112. [28] De KD, Whiting MF. Genetic and phylogeographic structure of populations of Pulex simulans (Siphonaptera) in Peru inferred from two genes (CytB and CoII) [J]. Parasitol Res, 2003, 91(1):55-59. [29] Lawrence AL, Brown GK, Peters B, et al. High phylogenetic diversity of the cat flea (Ctenocephalides felis) at two mitochondrial DNA markers[J]. Med Vet Entomol, 2014, 28(3):330-336. [30] Lawrence AL, Hii SF, Jirsová D, et al. Integrated morphological and molecular identification of cat fleas (Ctenocephalides felis) and dog fleas (Ctenocephalides canis) vectoring Rickettsia felis in central Europe[J]. Vet Parasitol, 2015, 210(3-4):215-223. [31] Chandra S, Forsyth M, Lawrence AL,et al. Cat fleas (Ctenocephalides felis) from cats and dogs in New Zealand: molecular characterisation, presence of Rickettsia felis and Bartonella clarridgeiae and comparison with Australia[J]. Vet Parasitol, 2017, 234(1):25-30. [32] 罗丹, 王安东, 尹小平, 等. 中哈边境阿拉山口口岸输入性猫栉首蚤指名亚种线粒体COⅠ和COⅡ基因序列分析[J]. 中国人兽共患病学报, 2016, 32(9):789-792.