Abstract：Objective To study the inhibitory effect of astragalus polysaccharide (APS) on the proliferation of HepG2 cells and its potential mechanism. Methods HepG2 cells were cultured and treated with 100 μg/ml, 200 μg/ml or 400 μg/ml APS. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the cell proliferation. Western blot was employed to examine the expression of glycogen synthase kinase 3β(GSK3β) . Results The absorbance at the 1st-7th day (D1-D7) in MTT assay and GSK3β expression in HepG2 cells at the 5th hour aftertreatment were significantly lower in the groups of APS100, APS200 and APS400 than in the control group(P<0.05). No statistically significant differences were found in the D1-D7 absorbance and GSK3β expression between the groups of APS100 and APS400 (P>0.05), but both of them were higher than those of the APS200 group (P<0.05). Conclusions Astragalus polysaccharide can significantly inhibit the proliferation of HepG2 cells, and its mechanism may be related to inhibiting GSK3β expression. The results suggest that 200 μg/ml ASP has the best inhibitory effect.
刘礼,袁昌劲,郭敬杰,余涛,吕秀玮,刘娇萍,赖晓晶. 黄芪多糖抑制HepG2细胞增殖及可能机制研究[J]. 实用预防医学, 2018, 25(4): 385-387.
LIU Li, YUAN Chang-jin, GUO Jing-jie, YU Tao, LYU Xiu-wei, LIU Jiao-ping, LAI Xiao-jing. Inhibitory effect of astragalus polysaccharide on the proliferation of HepG2 cells and its potential mechanism. , 2018, 25(4): 385-387.
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2018, Vol. 25 
