Abstract：ObjectiveTo investigate the role of X-linked inhibitor of apoptosis protein (XIAP) expression in lead induced mouse hippocampal neuron HT22 cell injury. ethods We established lead exposure cell model in mouse HT22 cells. Methyl thiazolyl tetrazolium (MTT) assay was performed to determine the best proliferation density of HT22 cells and viability alterations of HT22 cells exposed to lead acetate at 0~100 μM for 24h and 48h．The expression levels of XIAP in HT22 cells after lead exposure were detected by Western blot, and the expression and distribution of XIAP in HT22 cells were determined by immunofluorescence staining. ResultsMTT assay showed that cell proliferation was inhibited in HT22 cells by exposure to 5 and 10μM lead acetate for 48h（P<0.05 and P <0.01, respectively). Western blot showed that XIAP expression decreased in HT22 cells treated with 5 and 10μM lead acetate when compared with the controls (P < 0.05). Immunofluorescence staining showed that lead acetate exposure for 48h could significantly reduced the XIAP expression in HT22 cells. onclusions XIAP may be involved in the lead-induced mouse hippocampus neuron HT22 cell injury.
康蓓佩,赵芳,苏鹏,王迪雅,陈景元,曹子鹏. XIAP蛋白表达改变在铅诱导小鼠海马神经元HT22细胞损伤中的作用[J]. 实用预防医学, 2015, 22(5): 513-516.
KANG Bei-pei, ZHAO Fang, SU Peng, WANG Di-ya, CHEN Jing-yuan, CAO Zi-peng. Role of XIAP expression change in lead-induced mouse hippocampal neuron HT22 cell injury. , 2015, 22(5): 513-516.
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2015, Vol. 22 
