Abstract：MethodsThe influenza A(H3N2) virus were isolated with MDCK cells from 6 throat swab samples in Zhongshan City in 2010-2013. The virus RNA was extracted , and the hemagglutinin gene was amplified by RT-PCR and sequenced. The genetic evolutionary variations of H3N2 virus HA among different years and in contrast with the vaccine strains were analyzed by similarity retrieval using BLASTn. ResultsSix strains of influenza A(H3N2) virus were isolated, with 2 strains for each year. The strains circulating in Zhongshan City were on the same branch with most of the epidemic strains in other countries or regions in the same year. The longer the evolutionary time, the farther the evolutionary distance. Compared with the strains from 2010, 12 amino acid loci changed in the HA1 of the strains from 2012, three of which occurred in region B and C. Compared with the strains from 2012, 4 amino acid loci mutated in the HA1 of the strains from 2013, and two mutations were in region A. Compared with the vaccine strains, there were respectively 6 and 8 amino acid locus mutations in the HA1 of the strains from 2012 and 2013, and 2 and 4 mutations were in region A, B and C. The receptor binding sites didn’t changed. ConclusionsThere are variations in the epidemic strains of influenza A(H3N2) virus among different years in 2010-2013 in Zhongshan City. The evolutionary distance gets farther as the evolutionary time gets longer. The epidemic strains still vary from the vaccine strains.
吴衍恒,欧慧,师舞阳,谢颖,周日东. 2010-2013年中山市流感病毒(H3N2)血凝素基因特征分析[J]. 实用预防医学, 2015, 22(4): 420-423.
WU Yan-heng, OU Hui, SHI Wu-yang, XIE Ying, ZHOURi-dong. Analysis on the genetic characteristics of influenza A (H3N2) virus hemmagglutinin (HA) in Zhongshan City in 2010-2013. , 2015, 22(4): 420-423.
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