Influence of Broken-spores of Ganoderma lucidum Metallothionein expression on rat challenged by long-term cadmium exposure
Ge Zhen dan1, Huang Hou jin1, Zhu Bao shi2, Yang cheng2, Feng Yang3
1.Department of Toxicology,School of Public Health,Zunyi Medical University,Zunyi Guizhou 563099; 2. Department of Infection management, Yi chun First people’s Hospital, Yi chun Hei long jiang, 153000, China
Abstract：Objective To investigate influence of broken-spores of Ganoderma lucidum (GLS) to metallothionein expression on rat challenged by long-term cadmium exposure. Methods 80 male SD rats were randomly divided into four groups：control group(C),CdCl2 group (Cd) and two drug groups(0.5g/kg1.0 g/kg GLS plus CdCl2, namely GCdL and GCdH ). Rats were given intraperitioneal injection of 2mg/kg cadmium chloride (CdCl2) once every two days except control group, GCdL or GCdH groups were treated with GLS by oral administrations at doses of 0.5g/kg,1.0g/kg once a day respectively , C group and CdCl2 group were gavaged with isovolumetric distilled water. The rats’ manifestation was recognized during experiment and their hepatic tissues were taken on 30d, 60d, 90d respectively, the Cd contents in liver tissue subcelluarn nucleus, miochondria, microsome, cytoplasm were tested by Atomic Absorption Spectrophotometer on 90d, MT-1mRNAand MT-2 mRNA in hepatic tissue were determined by real time RT-PCR. Results Compared with the cadmium group alone, the control group and drug groups were statistically siginificant on the rats weight(P<0.05); Subcellular distribution of cadmium in the livers changed significantly(P<0.05); Compared with the control group, expression of MT-1mRNA and MT-2mRNA were increased in drug groups(P<0.05), and in time-dose effect manner. Conclusion GLS may be effective on preventing or treating Cd-induced rat hepatic damage for long-term cadmium exposure, and one of the underlying mechanisms may be related to MT-1mRNA and MT-2mRNA expressive level .
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Ge Zhen dan, Huang Hou jin, Zhu Bao shi, Yang cheng, Feng Yang. Influence of Broken-spores of Ganoderma lucidum Metallothionein expression on rat challenged by long-term cadmium exposure. , 2016, 23(4): 395-398.
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2016, Vol. 23 
