Abstract:Objective To develop a method for the determination of aflatoxins B1, B2, G1, and G2 by high performance liquid chromatography (HPLC) coupled with post-column photochemical derivatization. Methods The aflatoxins were extracted by acetonitrile-water solution, and purified by multifunctional clean-up columns. The extract was separated by HPLC with methanol-water isocratic elution, and quantified by fluorescence detection after photochemical derivatization. Results The baseline separation of aflatoxins B1, B2, G1 and G2 was achieved within 24 minutes. The detection limits of aflatoxins B1, G1 and aflatoxins B2, G2 were 0.2 μg/kg and 0.1 μg/kg, respectively. The average recovery of standard addition for spiked samples in 1.0-5.0μg/kg was 84.0%-94.6%, and the relative standard deviation was 3.4%-6.5%. Conclusions This method possesses several advantages, including high sensitivity, simplicity, precision and repeatability, and it is applicable to the batch analysis of aflatoxins.
宋月, 陈颖, 白欣. 光化学衍生-高效液相色谱法检测食品中的黄曲霉毒素[J]. 实用预防医学, 2016, 23(7): 882-884.
SONG Yue, CHEN Ying, BAI Xin. Determination of aflatoxins in food by high performance liquidchromatography coupled with post-column photochemical derivatization. , 2016, 23(7): 882-884.