Abstract：Objective To develop a method for simultaneous determination of 16 kinds of alkaloids including pyrrolizidine, Tripterygium wilfordii Hook. F., coriaria lactone and isoquinoline in honey samples by using ultra performance liquidchromatography-tandem mass spectrometry (UPLC-MS/MS). Methods The honey samples were extracted with 0.1% formic water, and then separated on a Waters Phenyl column by gradient elution methanol with 0.1% formic acid as mobile phase. The detection of alkaloids was performed by using electrospray ionization in positive ion mode (ESI+) with multiple reactions monitoring (MRM) mode. Quantification was performed using the matrix-matched calibration curves. Results The linearity of the calibration curves of all alkaloids was observed within a given linear range with good correlation coefficients (r≥0.99). The limits of quantification were 0.005-5 μg/kg. The average recoveries for alkaloids in honey samples at three different spiked concentration levels ranged from 60.5% to 117.5%, with relative standard deviations of 0.8%-8.7%. The method established was used to determine 40 honey samples, and berberine was detected in 15 samples, with the concentrations ranging from 0.05-4.61 μg/kg. Seneciophylline was contained in 3 samples, with the concentrations being 14.3 μg/kg, 17.6 μg/kg and 17.8 μg/kg respectively. Conclusion The method developed has the advantages of simple operation, high recoveries and good reproducibility, and it is suitable for simultaneous determination of alkaloids in honey.
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2023, Vol. 30 
