Abstract：Objective To investigate the relationship between the contamination status of norovirus in oyster farms, sediment and water samples, therelationship betweenwater temperature in oyster farms and detection rate of norovirus in Beihai City, Guangxi, so as to explore the correlation between oyster production environment and norovirus contamination and to put forward suggestions for taking effective measures to reduce norovirus contamination in oysters. Methods A total of 336 oyster, sediment and water samples were collected from 2 oyster farms in Beihai City, Guangxi between January and December in 2014, and water temperature at the sampling sites was simultaneously monitored. Real-time reverse transcription polymerase chain reaction (RT-PCR) assay was used to detect the presence of norovirus RNA and genogroups. Results The overall detection rate of norovirus in the 336 samples was 12.50% (42/336). The detection rates of norovirus in oyster and sediment samples were 15.83% (38/240) and 8.33%(4/48)respectively, and no norovirus was detected in water samples. Norovirus genotyping revealed that the detected noroviruses all belonged to genogroup II (GII). No statistically significant difference was observed in the detection rate of norovirus in oyster and sediment samples (P>0.05), but the detection rate of norovirus was significantly higher in oyster samples than in water samples (P<0.01). The overall detection rate of norovirus increased with the decrease of water temperature in the oyster farms. Conclusions The oysters and sediment in the oyster farms in Beihai City, Guangxi in 2014 are heavily contaminated with norovirus GII. The norovirus contamination in oyster and sediment is closely correlated with water temperature in the oyster farms.The correlation between norovirus contamination in oyster and in water environment at production sites needs further analysis.
吕素玲, 李秀桂, 姚雪婷, 诸葛石养. 2014年广西北海市养殖环境与牡蛎中诺如病毒污染相关性调查[J]. 实用预防医学, 2017, 24(7): 769-771.
LYU Su-ling, LI Xiu-gui, YAO Xue-ting, ZHEGE Shi-yang. Correlation between oyster production environment and norovirus contamination in Beihai City, Guangxi, 2014. , 2017, 24(7): 769-771.
2017, Vol. 24 
