Abstract:Objective To investigate the species, distribution and seasonal fluctuation of Anopheles mosquitoes in Xiuying district of Haikou city in 2018, to assess the risk of malaria transmission, and to provide a scientific basis for formulating prevention and control strategy and programs after malaria elimination. Methods We investigated the species and distribution of Anopheles caught by trapping with light traps, trapping animal-bait and trapping human-bait in six natural villages which were randomly selected from six towns in Xiuying district. One natural village was selected as a monitoring point to study the seasonal fluctuation of Anopheles mosquitoes. Results A total of 458 Anopheles mosquitoes, being to six species, were captured at six surveyed spots, including An. sinensis (47.60%, 218/458), An. tessellates (40.83%, 187/458), An. vagus (9.61%, 44/458), An. arbirostris (0.87%, 4/458), An. aconitus (0.66%, 3/458) and An. peditaeniatus (0.44%, 2/458). A total of 1,421 Anopheles mosquitoes, being to seven species, were captured at the monitoring point of seasonal fluctuation from January to December, including An. sinensis (44.76%), An. tessellates (39.55%) and An. vagus (15.20%). The number of Anopheles mosquitoes increased from April, and peaked in September. The number of Anopheles mosquitoes captured in May-November accounted for 89.23% of the whole year. An. sinensis was active all year round. ConclusionsAn. sinensis, An. tessellates and An. vagus were the predominant Anopheles mosquito species in Xiuying district of Haikou city in 2018, but An. minimus which was the major mosquito species of malaria vector in the past had not been identified. The density of Anopheles mosquitoes was continuously higher in May-November in 2018, but lower in other months.