Detection of pathogens in children with acute respiratory infection in Wuhan region
WU Ze-gang1, LI Zhi-qing2, GU Jian1, LI Yan1
1.Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China; 2.Tianyou Hospital Affiliated to Wuhan University of Science and Technology, Wuhan, Hubei 430064, China
Abstract：Objective To detect the viruses and atypical bacteria in acute respiratory infection (ARI) in children aged 15 years and below, and to investigate the prevalence of these pathogens in Wuhan region. Methods A total of 41,434 serum specimens were collected from inpatients who were intended to diagnose as ARI in Department of Pediatrics, Renmin Hospital of Wuhan University from September 2012 to August 2017. Indirect immunofluorescence assays were performed to detect IgM antibodies against nine kinds of common pathogens, including adenovirus (ADV), Coxiella burnetii (COX), Chamydophila pneumonia (CP), influenza A virus (FluA), influenza B virus (FluB), Legionella pneumophila 1 (LPN1), Mycoplasma pneumoniae (MP), parainfluenza virus (PIV) and respiratory syncytial virus (RSV). Results Of the 41,434 detected specimens, 20,373 (49.17%) were tested positive for at least one pathogen. The detection rate of MP was the highest (39.67%, 16,435/41,434), followed by FluB 7 (18.20%, 7,541/41,434) and PIV (5.54%, 2,297/41,434). FluB appeared a little popular in spring in 2013 and 2014. ADV was very prevalent in spring and summer in 2013 and 2014, PIV very prevalent in winter in 2013, in spring in 2014, in summer in 2016 and in spring and summer in 2017, and Cox prevalent in summer in 2013 and in autumn in 2014. FluA and LPN1 were a little popular in summer in 2013. Among the nine kinds of pathogens detected, the detection rates of ADV, CP, FluB, LPN1, MP, PIV and RSV showed statistically significant differences among different age groups (P<0.05). The total detection rate of pathogens was significantly lower in the under 1-year age group than in the other age groups (P<0.05). The patterns of mixed infections were complicated. In the mixed infection with 2 kinds of pathogens, MP+FluB was the most common pattern (8.82%, 3,656/41,434). Conclusions The most common pathogens causing ARI among children in Wuhan region were MP, FluB and PIV. The detection rate of each pathogen displayed specific seasonal variations and differences among different age groups. Mixed infections were more common than single infections, and the most common co-infection was MP+FluB.
吴泽刚, 黎知青, 顾剑, 李艳. 武汉地区儿童急性呼吸道感染的常见病原体检测[J]. 实用预防医学, 2019, 26(2): 133-137.
WU Ze-gang, LI Zhi-qing, GU Jian, LI Yan. Detection of pathogens in children with acute respiratory infection in Wuhan region. , 2019, 26(2): 133-137.
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2019, Vol. 26 
