Abstract：Objective To explore the effect of ozonated autohemotherapy (O3-AHT) on cellular immune function among patients with pulmonary tuberculosis. Methods Patients with active pulmonary tuberculosis hospitalized in Department of Surgery, Hunan Chest Hospital from January 2018 to February 2019 served as the study subjects. Following the informed consent process, eligible patients were randomly assigned to the study group (O3-AHT and antituberculosis treatment group) and the control group (antituberculosis medication alone group). The ratios of T-lymphocyte subsets (including CD4+, CD8+, CD4+/CD8+, CD45+, CD56+ and CD19+) in peripheral blood of patients in the two groups were detected before the treatment and 10 days after the treatment. The changes in various ratios before and after the treatment were compared within each group as well as between the two groups. Results A total of 133 patients with active pulmonary tuberculosis were enrolled in this study, including 71 patients in the study group and 62 in the control group. No statistically significant differences were found in the ratios of the T-lymphocyte subsets in peripheral blood before the treatment between the two groups (all P>0.05). After the treatment, statistically significant differences were observed in the ratios of CD4+, CD8+ and CD56+ T-lymphocyte subsets in peripheral blood between the two groups (all P<0.05), but no statistically significant differences were found in the ratios of CD4+/CD8+, CD19+ and CD45+ T-lymphocyte subsets in peripheral blood between the two groups (all P>0.05). In the study group, statistically significant differences were discovered in the ratios of CD4+, CD4+/CD8+, CD56+ and CD45+ T-lymphocyte subsets in peripheral blood between the pre- and post-treatment (all P<0.05), but no statistically significant differences were discovered in the ratios of CD8+ and CD19+ T-lymphocyte subsets between the pre- and post-treatment (both P>0.05). In the control group, there were statistically significant differences in the ratios of CD4+ and CD8+ T-lymphocyte subsets in peripheral blood between the pre- and post-treatment (both P<0.05), but no statistically significant differences were found in the ratios of CD4+/CD8+, CD45+, CD56+ and CD19+ T-lymphocyte subsets between the pre- and post-treatment (all P>0.05). Conclusion Ozonated autohemotherapy can improve the cellular immune function of patients with pulmonary tuberculosis.
石自力, 邓高焱, 白丽琼, 何超, 范明, 王勋, 王永利, 周月兰, 唐细良. 三氧自血疗法对肺结核患者细胞免疫功能的影响[J]. 实用预防医学, 2020, 27(1): 30-32.
SHI Zi-li, DENG Gao-yan, BAI Li-qiong, HE Chao, FAN Ming, WANG Xun, WANG Yong-li, ZHOU Yue-lan, TANG Xi-liang. Effect of ozonated autohemotherapy on cellular immune functionin patients with pulmonary tuberculosis. , 2020, 27(1): 30-32.
[1] Lu J, Ye S, Qin R, et al. Effect of Chinese herbal medicine extracts on cell-mediated immunity in a rat model of tuberculosis induced by multiple drug-resistant bacilli[J]. Mol Med Rep, 2013, 8(1):227-232. [2] 王永, 钱晓焱. 三氧自体血疗法专家共识[J]. 转化医学杂志, 2018, 7(6):10-12,29. [3] 高孟秋. 《WS 288-2017肺结核诊断》新标准中关于临床诊断患者判定的解析[J]. 中国防痨杂志, 2018,40(3):243-246. [4] 赵紫平, 陈安基.星状神经节阻滞对结核患者免疫功能的影响[J].实用预防医学, 2018, 25(10):1256-1257. [5] 庞增, 何日东, 陈勇军,等. 不同治疗时期肺结核患者T淋巴细胞亚群的动态变化及临床意义[J]. 现代医院, 2018, 18(4):543-545. [6] 唐神结,高文. 临床结核病学[Z].北京:人民卫生出版社,2011:66-70. [7] 张青,肖和平.T细胞、NKT细胞和NK细胞在结核病诊断中的意义探讨[J].中国防痨杂志,2010,32(5):267-271. [8] Yanaba K, Bouaziz JD, Haas KM, et al. A regulatory B cell subset with a unique CD1dhiCD5+ phenotype controls T cell-dependent inflammatory responses[J]. Immunity, 2008, 28(5):639-650. [9] Bocci V. Biological and clinical effects of ozone. Has ozone therapy a future in medicine?[J]. Br J Biomed Sci, 1999, 56(4):270-279. [10] Harkema JR, Plopper CG, Hyde DM, et al. Response of macaque bronchiolar epithelium to ambient concentrations of ozone[J]. Am J Pathol, 1993, 143(3):857-866. [11] 李丽, 张巧花, 侯淑玲,等. 三氧治疗对淋巴瘤患者T细胞亚群、NK细胞的影响[J]. 山西医科大学学报, 2007, 38(3):210-212.
2020, Vol. 27 
