Abstract：Objective To identify the polymorphism of alcohol dehydrogenase-1B (ADH1B) gene by polymerase chain reaction with confronting two-pair primers (PCR-CTPP) assay. Methods According to the sequence of ADH1B rs1229984, two pairs of primers were designed for polymerase chain reaction and the genotypes could be identified by electrophoresis, which were verified by DNA sequencing method. The distribution of ADH1B gene polymorphism was detected by PCR-CTPP in 58 cases of healthy people. Results PCR-CTPP method could be used for detecting ADH1B gene polymorphism accurately and the results of genotyping in PCR-CTPP were coincident with those performed by DNA sequencing. In 58 cases of healthy people, the ADH1B A allele was the dominant allele with the frequency of 71.6%. Conclusions The detection of ADH1B polymorphism by PCR-CTPP assay is simple, rapid, economical and labor-saving which can be widely applied to common laboratories for rapid screening and molecular epidemiological study of disease-related genes.
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2017, Vol. 24 
