Etiological surveillance results of hand, foot and mouth disease and genetic characteristics of VP1 from coxsackievirus A6 in Jinshan district of Shanghai, 2011-2018
SUN Qing-shuang, DONG Zhao-peng, ZANG Hao, DU Jian-ru, SONG Can-lei, LI Shu-hua
Jinshan District Center for Disease Control and Prevention, Shanghai 201599, China
Abstract:Objective To investigate the etiological features of hand, foot and mouth disease (HFMD) in Jinshan district of Shanghai from 2011 to 2018, to analyze the genetic characteristics of VP1 gene of coxsackievirus A6 (CoxA6), and to provide a theoretical basis for HFMD prevention and control. Methods The pathogenic detection results of specimens of HFMD surveillance in Jinshan district during 2011-2018 were statistically analyzed. CoxA6 virus isolates were sequenced for VP1 nucleotide sequence, and homologous diseases were analyzed to construct phylogenetic tree. Results A total of 1,207 specimens of HFMD were collected in Jinshan district from 2011 to 2018. 703 cases were positive for etiology, with the positive rate of 58.24% (703/1,207). The main pathogenic viruses were CoxA6 (36.13%, 254/703), CoxA16 (30.87%, 217/703), EV71 (23.04%, 162/703), CoxA10 (2.56%, 18/703) and other enteroviruses (7.40%, 52/703). EV71, CoxA16 and other enteroviruses were the dominant pathogens in 2011-2013. CoxA6 gradually became the dominant pathogen from 2014, accounting for 85.43% of the total pathogenic composition in 2018. The incidence of EV71 showed a single peak distribution, and the peak appeared in May. CoxA16 showed a double peak distribution, the main peak appeared in June and the second peak in November. CoxA6 showed a double peak distribution, the main peak appeared in November and the second peak in July. Results from the monitoring point indicated that CoxA6 type appeared in 2013, whereas results from the epidemic monitoring showed that CoxA6 type appeared in 2014. Results from the monitoring point displayed that the age distribution of EV71, CoxA16 and CoxA6 pathogens was mainly concen-trated in 1-5 years old, of which CoxA6 was mainly concentrated in 7-12 years old. Results from the epidemic surveillance displayed that the distribution of EV71, CoxA16 and CoxA6 pathogens was mainly concentrated in primary classes and middle classes, of which CoxA6 was mainly concentrated in primary schools. Genetic analysis indicated that the CoxA6 pathogen gene prevalent in Jinshan district in 2017-2018 was characterized by the E2 subtype. Conclusions The dominant pathogen of HFMD in Jinshan district in 2011-2018 had been transferred from EV71 and CoxA16 to CoxA6 E2. Pathogen surveillance should be further strengthened, especially in the 7-12 year-old group. New pathogens should be found in time so as to provide a basis for implementing comprehensive prevention and control measures against HFMD.
孙箐爽, 董兆鹏, 臧昊, 杜荐如, 宋灿磊, 李淑华. 2011—2018年上海市金山区手足口病病原学监测结果及柯萨奇病毒A6型分离株VP1基因特征分析[J]. 实用预防医学, 2020, 27(1): 33-37.
SUN Qing-shuang, DONG Zhao-peng, ZANG Hao, DU Jian-ru, SONG Can-lei, LI Shu-hua. Etiological surveillance results of hand, foot and mouth disease and genetic characteristics of VP1 from coxsackievirus A6 in Jinshan district of Shanghai, 2011-2018. , 2020, 27(1): 33-37.